Tomb J F, White O, Kerlavage A R, Clayton R A, Sutton G G, Fleischmann R D, Ketchum K A, Klenk H P, Gill S, Dougherty B A, Nelson K, Quackenbush J, Zhou L, Kirkness E F, Peterson S, Loftus B, Richardson D, Dodson R, Khalak H G, Glodek A, McKenney K, Fitzegerald L M, Lee N, Adams M D, Hickey E K, Berg D E, Gocayne J D, Utterback T R, Peterson J D, Kelley J M, Cotton M D, Weidman J M, Fujii C, Bowman C, Watthey L, Wallin E, Hayes W S, Borodovsky M, Karp P D, Smith H O, Fraser C M, Venter J C
Institute for Genomic Research, Rockville, Maryland 20850, USA.
Nature. 1997 Aug 7;388(6642):539-47. doi: 10.1038/41483.
Helicobacter pylori, strain 26695, has a circular genome of 1,667,867 base pairs and 1,590 predicted coding sequences. Sequence analysis indicates that H. pylori has well-developed systems for motility, for scavenging iron, and for DNA restriction and modification. Many putative adhesins, lipoproteins and other outer membrane proteins were identified, underscoring the potential complexity of host-pathogen interaction. Based on the large number of sequence-related genes encoding outer membrane proteins and the presence of homopolymeric tracts and dinucleotide repeats in coding sequences, H. pylori, like several other mucosal pathogens, probably uses recombination and slipped-strand mispairing within repeats as mechanisms for antigenic variation and adaptive evolution. Consistent with its restricted niche, H. pylori has a few regulatory networks, and a limited metabolic repertoire and biosynthetic capacity. Its survival in acid conditions depends, in part, on its ability to establish a positive inside-membrane potential in low pH.
幽门螺杆菌26695菌株拥有一个由1667867个碱基对组成的环状基因组，预测有1590个编码序列。序列分析表明，幽门螺杆菌具有完善的运动系统、铁清除系统以及DNA限制与修饰系统。鉴定出了许多假定的黏附素、脂蛋白和其他外膜蛋白，这突出了宿主-病原体相互作用的潜在复杂性。基于大量编码外膜蛋白的序列相关基因以及编码序列中存在同聚物序列和二核苷酸重复序列，幽门螺杆菌与其他几种黏膜病原体一样，可能利用重复序列内的重组和滑链错配作为抗原变异和适应性进化的机制。与其受限的生态位一致，幽门螺杆菌拥有少数调控网络，代谢能力有限，生物合成能力也有限。它在酸性条件下的存活部分取决于其在低pH值下建立膜内正电位的能力。
