Hiraoka A, Kawakami M, Fukuda H, Nakamura H, Karasuno T, Teshima H, Masaoka T
Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
J Allergy Clin Immunol. 1997 Dec;100(6 Pt 2):S70-2. doi: 10.1016/s0091-6749(97)70008-3.
Among patients undergoing allogeneic bone marrow transplantation, we previously detected an increase of CD8+S6F1+ and CD8+CD57- cells with the onset of acute graft-versus-host disease.
This study was an attempt to develop a simple laboratory test for graft-versus-host disease.
We analyzed the percentage of the two lymphocyte subsets in the peripheral blood mononuclear cells of healthy volunteers, patients with posttransfusion graft-versus-host disease, and recipients of allogeneic bone marrow transplants.
Two patients with posttransfusion graft-versus-host disease showed a high percentage of both subsets. When the graft-versus-host disease pattern was defined as 45% or more CD8+S6F1+ cells and 35% or more CD8+CD57- cells, it was found in none of 17 recipients without acute graft-versus-host disease, 9 of 16 recipients with grade I disease, and 8 of 9 recipients with grade II or worse disease had this pattern.
Our test may be useful for the laboratory diagnosis of acute graft-versus-host disease.
在接受异基因骨髓移植的患者中，我们之前检测到随着急性移植物抗宿主病的发生，CD8+S6F1+和CD8+CD57-细胞增多。
本研究试图开发一种用于移植物抗宿主病的简单实验室检测方法。
我们分析了健康志愿者、输血后移植物抗宿主病患者以及异基因骨髓移植受者外周血单个核细胞中这两种淋巴细胞亚群的百分比。
两名输血后移植物抗宿主病患者的这两个亚群百分比均较高。当将移植物抗宿主病模式定义为CD8+S6F1+细胞占45%或更多且CD8+CD57-细胞占35%或更多时，17名无急性移植物抗宿主病的受者中无一例出现这种模式，16名I级疾病受者中有9例出现，9名II级或更严重疾病受者中有8例出现。
我们的检测方法可能有助于急性移植物抗宿主病的实验室诊断。
