Gryglewski A, Szczepanik M, Majcher P, Popiela T, Ptak W
First Department of General and Gastrointestinal Surgery, Jagiellonian University College of Medicine, Kraków, Poland.
J Surg Res. 1997 Dec;73(2):137-42. doi: 10.1006/jsre.1997.5220.
Stress, including surgical trauma, results in different dysfunctions of the body. In our former experiments on posttraumatic modification of immune response of gastrectomized mice we observed a significant suppression of contact sensitivity. This could be transferred by lymph nodes and spleen T lymphocytes of mice which underwent surgery.
We studied changes in gammadelta and alphabeta T cell numbers in peripheral blood, Peyer's patches, and mesenteric lymph nodes after partial gastrectomy (major operation) and after sham gastrectomy (laparotomy-minor operation) in mice. The number of gammadelta and alphabeta T cells was counted on the FACSTAR cell sorter before and 1, 2, 3, 7, and 14 days after surgery.
In our observations there was a significant increase of percentage of gammadelta T cells both in Peyer's patches (1.9 +/- 0.5 to 10.5 +/- 0.3) and in mesenteric lymph nodes (2.7 +/- 0.7 to 8.8 +/- 3.5) on the third day after partial gastrectomy (546 and 322% of control values, respectively). In contrast in gastrectomized mice the number of alphabeta T cells in Peyer's patches (38.4 +/- 6.8 to 21.2 +/- 6.2) and lymph nodes (56.7 +/- 15 to 40.4 +/- 17) was decreased on day 3 (55 and 71% of control values, respectively). There was a decline in both alphabeta (18.1 +/- 8 to 8.2 +/- 1.7) and gammadelta (6.7 +/- 2.8 to 3.9 +/- 2) T cell numbers in peripheral blood on days 1, 3, and 7 (45 and 58% of normal values, respectively). Sham operation had no significant influence on alphabeta and gammadelta T cell numbers. We observed that after leg amputation the number of gammadelta T cells in mesenteric lymph nodes was significantly increased on day 3 (0.8 +/- 0.2 to 7.7 +/- 0.3), 770% of normal. In contrast to this, leg amputation had a negligible effect on T cell counts in Peyer's patches during all periods of observation.
We suggest that the major surgical stress (partial gastrectomy) may disturb the normal cell traffic selectively with increased gammadelta T cell homing in intestinal Peyer's patches and lymph nodes (GALT) and with the cell displacement from peripheral blood to lymphatic organs. The severity and localization of stress may be crucial.
压力，包括手术创伤，会导致身体出现不同的功能障碍。在我们之前关于胃切除小鼠创伤后免疫反应改变的实验中，我们观察到接触敏感性受到显著抑制。这种抑制可通过接受手术的小鼠的淋巴结和脾脏T淋巴细胞传递。
我们研究了小鼠部分胃切除（大手术）和假胃切除（剖腹术 - 小手术）后外周血、派尔集合淋巴结和肠系膜淋巴结中γδ和αβ T细胞数量的变化。在手术前以及手术后1、2、3、7和14天，使用FACSTAR细胞分选仪对γδ和αβ T细胞数量进行计数。
在我们的观察中，部分胃切除术后第三天，派尔集合淋巴结中γδ T细胞百分比显著增加（从1.9±0.5增至10.5±0.3），肠系膜淋巴结中γδ T细胞百分比也显著增加（从2.7±0.7增至8.8±3.5），分别为对照值的546%和322%。相比之下，胃切除小鼠的派尔集合淋巴结（从38.4±6.8降至21.2±6.2）和淋巴结（从56.7±15降至40.4±17）中的αβ T细胞数量在第3天减少，分别为对照值的55%和71%。在第1、3和7天，外周血中αβ（从18.1±8降至8.2±1.7）和γδ（从6.7±2.8降至3.9±2） T细胞数量均下降，分别为正常值的45%和58%。假手术对αβ和γδ T细胞数量无显著影响。我们观察到截肢后第三天肠系膜淋巴结中γδ T细胞数量显著增加（从0.8±0.2增至7.7±0.3），为正常值的770%。与此相反，在所有观察期间，截肢对派尔集合淋巴结中的T细胞计数影响可忽略不计。
我们认为，重大手术应激（部分胃切除）可能会选择性地扰乱正常的细胞迁移，导致γδ T细胞在肠道派尔集合淋巴结和淋巴结（肠道相关淋巴组织）中的归巢增加，以及细胞从外周血向淋巴器官的转移。应激的严重程度和部位可能至关重要。
