碘化4S、5S以及18S + 28S RNA与蝾螈染色体的分子杂交
Molecular hybridization of iodinated 4S, 5S, and 18S + 28S RNA to salamander chromosomes.
作者信息
León P E
出版信息
J Cell Biol. 1976 May;69(2):287-300. doi: 10.1083/jcb.69.2.287.
4S, 5S, AND 18S + 28S RNA from the newt Taricha granulosa granulosa were iodinated in vitro with carrier-free 125I and hybridized to the denatured chromosomes of Taricha granulosa and Batrachoseps weighti. Iodinated 18S + 28S RNA hybridizes to the telomeric region on the shorter arm of chromosome 2 and close to the centromere on the shorter arm of chromosome 9 from T. granulosa. On this same salamander the label produced by the 5S RNA is located close to or on the centromere of chromosome 7 and the iodinated 4S RNA labels the distal end of the longer arm of chromosome 5. On the chromosomes of B. wrighti, 18S + 28S RNA hybridizes close to the centromeric region on the longer arm of the largest chromosome. Two centromeric sites are hybridized by the iodinated 5S RNA. After hybridization with iodinated 4S RNA, label is found near the end of the shorter arm of chromosome 3. It is concluded that both ribosomal and transfer RNA genes are clustered in the genome of these two salamanders.
用无载体的¹²⁵I对粗皮蝾螈（Taricha granulosa granulosa）的4S、5S以及18S + 28S RNA进行体外碘化，并使其与粗皮蝾螈和威氏钝口螈（Batrachoseps weighti）的变性染色体杂交。碘化的18S + 28S RNA与粗皮蝾螈第2号染色体短臂的端粒区域以及第9号染色体短臂靠近着丝粒的位置杂交。在同一种蝾螈中，5S RNA产生的标记位于第7号染色体着丝粒附近或其上，而碘化的4S RNA标记第5号染色体长臂的远端。在威氏钝口螈的染色体上，18S + 28S RNA与最大染色体长臂靠近着丝粒区域的位置杂交。碘化的5S RNA与两个着丝粒位点杂交。用碘化的4S RNA杂交后，在第3号染色体短臂末端附近发现标记。得出的结论是，核糖体RNA基因和转移RNA基因在这两种蝾螈的基因组中都是成簇分布的。
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