Moser J, Meyers K M, Russon R H, Reeves J J
Department of Pharmacology/Toxicology, Washington State University, Pullman 99164-6510, USA.
Am J Vet Res. 1998 Jan;59(1):111-8.
To determine whether plasma von Willebrand factor (vWf) concentration changes during the estrous cycle and pregnancy in Doberman Pinschers with type-I von Willebrand's disease (vWd) and in mixed-breed dogs with normal vWf, and if so, whether alterations in vWf concentration are associated with changes in serum concentrations of reproductive hormones.
5 sexually intact female Doberman Pinschers with type-I vWf and 8 sexually intact female mixed-breed dogs with normal vWf.
Concentrations of plasma vWf and serum progesterone and estradiol-17 beta were measured during the estrous cycle of nonpregnant dogs and during pregnancy, parturition, and lactation. Serum concentrations of total triiodothyronine, total thyroxin, and free thyroxin were measured during pregnancy, parturition, and lactation.
Von Willebrand factor concentration did not change during the estrous cycle, but during pregnancy, vWf concentration gradually increased. Peak concentrations were obtained at parturition and were 103 and 92% higher in mixed-breed dogs and dogs with type-I vWd, respectively, than were mean prepregnancy (anestrus) values. At parturition, total triiodothyronine concentration decreased from the prepregnancy value. The increase in vWf concentration during pregnancy was positively associated with changes in concentration of estradiol-17 beta and was negatively associated with changes in concentration of progesterone.
The increase in vWf concentration in pregnant bitches may be associated with changes in concentrations of reproductive hormones. However, the increase in vWf concentration during pregnancy may involve other factors because vWf concentration did not change during the estrous cycle of nonpregnant dogs despite increases in concentrations of estradiol-17 beta and progesterone.
确定患有I型血管性血友病（vWd）的杜宾犬以及血管性血友病因子（vWf）正常的混血犬在发情周期和妊娠期血浆vWf浓度是否发生变化，若有变化，vWf浓度的改变是否与生殖激素血清浓度的变化相关。
5只患有I型vWf的性成熟雌性杜宾犬和8只vWf正常的性成熟雌性混血犬。
在未怀孕母犬的发情周期以及妊娠期、分娩期和哺乳期测量血浆vWf、血清孕酮和雌二醇-17β的浓度。在妊娠期、分娩期和哺乳期测量血清总三碘甲状腺原氨酸、总甲状腺素和游离甲状腺素的浓度。
vWf浓度在发情周期中未发生变化，但在妊娠期，vWf浓度逐渐升高。在分娩时达到峰值浓度，混血犬和患有I型vWd的犬分别比妊娠前（发情间期）平均浓度高103%和92%。在分娩时，总三碘甲状腺原氨酸浓度从妊娠前的值下降。妊娠期vWf浓度的升高与雌二醇-17β浓度的变化呈正相关，与孕酮浓度的变化呈负相关。
怀孕母犬vWf浓度的升高可能与生殖激素浓度的变化有关。然而，妊娠期vWf浓度的升高可能涉及其他因素，因为尽管雌二醇-17β和孕酮浓度升高，但未怀孕母犬发情周期中vWf浓度并未改变。
