Sculean A, Donos N, Reich E, Karring T, Brecx M
Department of Periodontology and Conservative Dentistry, University of the Saarland, Homburg, Germany.
J Periodontal Res. 1998 Nov;33(8):453-9. doi: 10.1111/j.1600-0765.1998.tb02344.x.
The aim of the present study was to investigate in monkeys the regrowth of oxytalan fibres in different types of acute and chronic periodontal defects following regenerative periodontal treatment. One-wall intrabony and mandibular furcation III-defects were produced surgically in 3 monkeys (Macaca fascicularis). After a 6-wk dental plaque accumulation period the defects were exposed using a full thickness flap procedure. The granulation tissue was removed and the root surfaces were scaled and planed. Additionally, fenestration-type defects were produced at the vestibular surfaces of the maxillary and mandibular canines by surgically removing the vestibular bone plates and the root cementum. Subsequently, the defects were treated with guided tissue regeneration (GTR), enamel matrix proteins (EMP), combination of EMP and GTR or with coronally repositioned flaps. The postoperative care included tooth cleaning once a week during the experiment. After 5 months the animals were sacrificed and and the block sections were embedded in paraffin. Eight microns histological sections were cut and stained with the oxone-aldehyde-fuchsin-Halmi method. The results revealed that in all specimens where a regenerated periodontal ligament could be observed newly formed oxytalan fibers were present. They had a mainly apico-occlusal orientation and were localized closer to the cementum than to the alveolar bone. The regenerated oxytalan fibers had a similar morphological appearance than those observed in the original periodontal ligament regardless of the treatment modality by which regeneration was accomplished. Their presence was related to that of newly formed cementum suggesting a strong relationship between these 2 tissues. The neoformation of oxytalan fibres can thus be observed in some types of periodontal defects where the cementum and the periodontal ligament have been regenerated.
本研究的目的是在猴子身上研究再生性牙周治疗后不同类型急性和慢性牙周缺损中氧化还原纤维的再生情况。在3只猕猴（食蟹猴）身上通过手术制造了单壁骨内和下颌Ⅲ度根分叉缺损。经过6周的牙菌斑堆积期后，使用全厚瓣手术暴露缺损部位。去除肉芽组织，对牙根表面进行刮治和根面平整。此外，通过手术去除上颌和下颌犬齿前庭骨板和牙根牙骨质，在前庭表面制造开窗型缺损。随后，对缺损部位进行引导组织再生（GTR）、釉基质蛋白（EMP）、EMP与GTR联合治疗或冠向复位瓣治疗。术后护理包括在实验期间每周清洁一次牙齿。5个月后处死动物，将块状切片石蜡包埋。切取8微米厚的组织切片，采用过氧酸-醛-品红-哈尔米法染色。结果显示，在所有可观察到再生牙周膜的标本中均存在新形成的氧化还原纤维。它们主要呈根尖向咬合方向排列，且更靠近牙骨质而非牙槽骨。无论通过何种治疗方式实现再生，再生的氧化还原纤维与原始牙周膜中观察到的纤维具有相似的形态外观。它们的存在与新形成的牙骨质相关，表明这两种组织之间存在密切关系。因此，在一些牙骨质和牙周膜已再生的牙周缺损类型中可以观察到氧化还原纤维的新生。
