Ivarsson M, Ebenfelt A, Lundberg C
Department of Otorhinolaryngology, Head and Neck Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
Acta Otolaryngol. 1997 Nov;117(6):878-82. doi: 10.3109/00016489709114218.
To elucidate whether leukocytes in the surface secretion on the adenoid have a potential immunological function, imprint samples of surface secretion were obtained from the adenoid of 11 children and the corresponding mucosal area of 10 adults. May-Grünewald-Giemsa staining was used for evaluation of cell morphology and spatial relations, and immunohistochemical staining for identification of granulocytes, B cells, T cells and macrophages. The children's imprints showed large numbers of leukocytes, with mononuclear cells in the majority. The adults' imprints were characterized by moderate numbers of epithelial cells and few leukocytes. In six out of seven adenoid secretions successfully analysed with all four CD antigens, there was the simultaneous presence of granulocytes, B cells, 1 cells and macrophages. This was the case in one of nine analysable adult secretions. The CD-positive cells were often seen in juxtaposition, in clusters of two to 10 cells, as well as in contact with leukocytes of unknown CD antigenicity and epithelial cells. The simultaneous presence in the secretion of morphologically intact and CD antigen-presenting leukocytes in juxtaposition could indicate a potential immunological function of these cells.
为阐明腺样体表面分泌物中的白细胞是否具有潜在免疫功能，从11名儿童的腺样体及10名成人的相应黏膜区域获取了表面分泌物的印记样本。采用May-Grünewald-Giemsa染色评估细胞形态和空间关系，采用免疫组织化学染色鉴定粒细胞、B细胞、T细胞和巨噬细胞。儿童的印记显示有大量白细胞，其中单核细胞占多数。成人的印记特征为上皮细胞数量适中，白细胞较少。在成功用所有四种CD抗原分析的七份腺样体分泌物中，有六份同时存在粒细胞、B细胞、T细胞和巨噬细胞。九份可分析的成人分泌物中有一份是这种情况。CD阳性细胞常并列出现，形成2至10个细胞的簇，也与CD抗原性未知的白细胞和上皮细胞接触。分泌物中形态完整且呈递CD抗原的白细胞并列存在，可能表明这些细胞具有潜在免疫功能。
