Intraembryonic hematopoietic stem cells.

Intraembryonic hematopoietic stem cells (HSC) were first detected in avian chimeras associating an embryo with a yolk sac (YS). Cell markers were used to construct chimeras. The results showed that YS blood precursors undergo primitive erythropoiesis and become extinct, whereas intraembryonic precursors colonize rudiments of blood-forming organs and settle in the bone marrow as self-renewable HSC. The model is valid in the mouse as shown by in vitro cultures of cells obtained from embryo structures or YS separated prior to circulation. This approach, as well as restoration of irradiated adults, demonstrates that YS precursors have a limited potential compared with embryo precursors. The emergence of hematopoietic precursors in both YS and embryos is closely linked to the emergence of the endothelial network and is restricted to the mesoderm layer associated with endoderm.