Lessard E, Fortin A, Coquet A, Bélanger P M, Hamelin B A, Turgeon J
Faculty of Pharmacy, Laval University and Quebec Heart Institute, Laval Hospital, Sainte-Foy, Canada.
J Chromatogr Sci. 1998 Jan;36(1):49-54. doi: 10.1093/chromsci/36.1.49.
An improved high-performance liquid chromatographic assay for the determination of procainamide and N-acetylprocainamide (NAPA) at concentrations observed up to 32 h after a single oral dose administration of procainamide to human subjects is reported. Following liquid-liquid extraction of plasma samples, procainamide, NAPA, and the internal standard (N-propionylprocainamide) are separated on a reversed-phase C8 column with retention times of 4.0, 6.7, and 13.2 min, respectively. The ultraviolet detection limit (wavelength, 280 nm) of procainamide and NAPA is 2 ng/mL (signal-to-noise ratio, 3:1), and the quantitation limit is 4 ng/mL (signal-to-noise ratio, 5:1). Intra- and interday coefficients of variation are less than 8% in the range of 20-500 ng/mL.
据报道，一种改进的高效液相色谱法可用于测定人体单次口服普鲁卡因胺后长达32小时观察到的浓度下的普鲁卡因胺和N - 乙酰普鲁卡因胺（NAPA）。血浆样品经液 - 液萃取后，普鲁卡因胺、NAPA和内标（N - 丙酰普鲁卡因胺）在反相C8柱上分离，保留时间分别为4.0、6.7和13.2分钟。普鲁卡因胺和NAPA的紫外检测限（波长280nm）为2ng/mL（信噪比3:1），定量限为4ng/mL（信噪比5:1）。在20 - 500ng/mL范围内，日内和日间变异系数小于8%。
