Siragusa P, Tavazzi B, Lazzarino G, Di Pierro D, Belli A, Amorini M, Giuffrè R, Vagnozzi R
Department of Public Health, University of Rome Tor Vergata, Italy.
J Neurosurg Sci. 1997 Sep;41(3):249-55.
In this experimental study is illustrated an original model of cerebral asymmetric ischemia and reperfusion in the rat, induced by unilaterally elevating ICP and clamping the corresponding common carotid artery, that allows a direct comparison of the two brain hemispheres, one normal and the other ischemic, of the same animal.
The experimental procedure consisted in grafting two screws through the skull on the right side of the sagittal suture, one of them being connected to a Queckenstedt manometer for monitoring ICP variations. A nitroprusside solution (1 mg/ml administered through the femoral vein at a flow rate of 0.103 ml/min) was infused to achieve a significant drop of MABP. At this time point, animals were subjected to 5 min of ischemia and 10 min of reperfusion induced by clamping and declamping the right common carotid artery. During the whole period of ischemia and reperfusion ICP and MABP were constantly monitored. In order to provide an outlook on the metabolic alterations of brain tissue occurring during ischemia and reperfusion phenomena, several biochemical parameters of cellular energy metabolism and of oxygen radical-induced membrane damage were determined by a sensitive and reproducible HPLC method on perchloric acid tissue extracts.
The validity of the present model was supported by the finding of significant intrahemispheric differences in the concentration of several compounds considered as biochemical markers of tissue injury, such as adenosine 5'-triphosphate catabolites and malondialdehyde, this last indicating the damaging action of oxygen free radicals on cell membrane phospholipids.
在本实验研究中，阐述了一种大鼠脑不对称缺血再灌注的原始模型，该模型通过单侧升高颅内压（ICP）并夹闭相应的颈总动脉来诱导，它能够直接比较同一动物的两个脑半球，一个正常，另一个缺血。
实验步骤包括通过颅骨在矢状缝右侧植入两个螺钉，其中一个连接到奎肯施泰特压力计以监测颅内压变化。输注硝普钠溶液（以0.103 ml/min的流速通过股静脉给予1 mg/ml）以显著降低平均动脉压（MABP）。在这个时间点，通过夹闭和松开右侧颈总动脉诱导动物缺血5分钟和再灌注10分钟。在整个缺血和再灌注期间，持续监测颅内压和平均动脉压。为了了解缺血和再灌注现象期间脑组织发生的代谢改变，通过灵敏且可重复的高效液相色谱法对高氯酸组织提取物测定细胞能量代谢和氧自由基诱导的膜损伤的几个生化参数。
本模型的有效性得到了以下发现的支持：在几种被视为组织损伤生化标志物的化合物浓度方面，半球内存在显著差异，如5'-三磷酸腺苷分解代谢产物和丙二醛，后者表明氧自由基对细胞膜磷脂的损伤作用。
