Tanaka M, Dykes P J, Marks R
Department of Dermatology, University of Wales College of Medicine, Cardiff, UK.
Keio J Med. 1997 Dec;46(4):184-7. doi: 10.2302/kjm.46.184.
Human granulocyte-macrophage colony stimulating factor (GM-CSF) is a 22 kD glycoprotein derived from activated T cells, endothelial cells, fibroblasts and macrophages. It stimulates the proliferation and differentiation of haematopoietic cells and certain nonhaematopoietic cells. This study investigated the effect of GM-CSF on cultured human keratinocytes and determined whether these cells express GM-CSF receptors. Measurement of keratinocyte growth by image analysis showed that GM-CSF mildly stimulated the growth of keratinocytes. With 4 ng/ml GM-CSF the growth of primary keratinocytes and subcultured keratinocytes was only stimulated up to 25% and 18% of the control cell level, respectively. Human keratinocytes were incubated with GM-CSF at 4 ng/ml for 4 days to induce receptor expression. These cells showed only a weak positive reaction on affinity labelling using digoxigenated GM-CSF. We conclude that keratinocyte growth may be stimulated by GM-CSF but that the presence of GM-CSF receptors on these cells is equivocal.
人粒细胞巨噬细胞集落刺激因子（GM-CSF）是一种22 kD的糖蛋白，由活化的T细胞、内皮细胞、成纤维细胞和巨噬细胞产生。它刺激造血细胞和某些非造血细胞的增殖与分化。本研究调查了GM-CSF对培养的人角质形成细胞的影响，并确定这些细胞是否表达GM-CSF受体。通过图像分析测量角质形成细胞生长情况，结果显示GM-CSF轻度刺激角质形成细胞的生长。使用4 ng/ml的GM-CSF时，原代角质形成细胞和传代培养的角质形成细胞的生长分别仅被刺激至对照细胞水平的25%和18%。将人角质形成细胞与4 ng/ml的GM-CSF孵育4天以诱导受体表达。这些细胞在用洋地黄毒苷标记的GM-CSF进行亲和标记时仅显示弱阳性反应。我们得出结论，GM-CSF可能刺激角质形成细胞生长，但这些细胞上GM-CSF受体的存在尚不明确。
