Institute of Outcomes Research, Maria Sklodowska-Curie Medical Academy, 03-411 Warsaw, Poland.
Faculty of Medical Sciences and Health Sciences, Kazimierz Pulaski University of Radom, 26-600 Radom, Poland.
Int J Mol Sci. 2023 Dec 19;25(1):30. doi: 10.3390/ijms25010030.
Wounds represent a common occurrence in human life. Consequently, scientific investigations are underway to advance wound healing methodologies, with a notable focus on dressings imbued with biologically active compounds capable of orchestrating the wound microenvironment through meticulously regulated release mechanisms. Among these bioactive agents are cytokines, which, when administered to the wound milieu without appropriate protection, undergo rapid loss of their functional attributes. Within the context of this research, we present a method for fabricating dressings enriched with G-CSF (granulocyte colony-stimulating factor) or GM-CSF (granulocyte-macrophage colony-stimulating factor), showcasing both biological activity and protracted release dynamics. Based on Ligasano, a commercial polyurethane foam dressing, and chitosan crosslinked with TPP (sodium tripolyphosphate), these dressings are noncytotoxic and enable cytokine incorporation. The recovery of cytokines from dressings varied based on the dressing preparation and storage techniques (without modification, drying, freeze-drying followed by storage at 4 °C or freeze-drying followed by storage at 24 °C) and cytokine type. Generally, drying reduced cytokine levels and their bioactivity, especially with G-CSF. The recovery of G-CSF from unmodified dressings was lower compared to GM-CSF (60% vs. 80%). In summary, our freeze-drying approach enables the storage of G-CSF or GM-CSF enriched dressings at 24 °C with minimal cytokine loss, preserving their biological activity and thus enhancing future clinical availability.
伤口在人类生活中很常见。因此，科学界正在研究如何推进伤口愈合方法，其中一个重点是开发具有生物活性的敷料，这些敷料中含有能够通过精心调控的释放机制来调节伤口微环境的化合物。这些生物活性物质包括细胞因子，如果在没有适当保护的情况下将其施用于伤口环境中，它们的功能属性会迅速丧失。在本研究中，我们提出了一种制备富含 G-CSF（粒细胞集落刺激因子）或 GM-CSF（粒细胞-巨噬细胞集落刺激因子）的敷料的方法，展示了它们的生物活性和延长释放动力学。这些敷料基于 Ligasano（一种商业聚氨酯泡沫敷料）和用 TPP（三聚磷酸钠）交联的壳聚糖，具有非细胞毒性并能够掺入细胞因子。细胞因子从敷料中的回收情况因敷料的制备和储存技术（未经修饰、干燥、冷冻干燥后在 4°C 下储存或冷冻干燥后在 24°C 下储存）和细胞因子类型而异。一般来说，干燥会降低细胞因子的水平及其生物活性，尤其是 G-CSF。未经修饰的敷料中 G-CSF 的回收量低于 GM-CSF（60%对 80%）。总之，我们的冷冻干燥方法可实现以 24°C 储存富含 G-CSF 或 GM-CSF 的敷料，同时最小化细胞因子损失，保持其生物活性，从而提高未来的临床可用性。
