Stevenson P G, Doherty P C
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
J Virol. 1998 Feb;72(2):943-9. doi: 10.1128/JVI.72.2.943-949.1998.
Respiratory infection of BALB/c mice with the murine gammaherpesvirus 68 (MHV-68) induces the clonal expansion of virus-specific cytotoxic T-lymphocyte (CTL) precursors (CTLp) in the regional, mediastinal lymph nodes (MLN). Some of these CTLps differentiate to become fully functional CTL effectors, which can be detected in both the lymphoid tissue and in the site of pathology in the lung. Though the lymph nodes and spleen harbor substantial populations of latently infected B cells for life, the level of virus-specific CTL activity decreases rapidly in all sites. The CD8+ CTLp numbers fall to background levels in the MLN within several months of the termination of the productive phase of MHV-68 infection in the respiratory epithelium but are maintained at relatively low frequency in the spleen. The continued presence of a gamma interferon-producing, MHV-68-specific CD4+ set can also be demonstrated in cultured spleen cells. The virus-specific immunoglobulin G (IgG) response is slow to develop, with serum neutralizing antibody and enzyme-linked immunosorbent assay titers continuing to rise for several months. The level of total serum IgG increases dramatically within 2 weeks of infection, probably as a consequence of polyclonal B-cell activation, and remains high. The immune response profile is clearly influenced by the persistence of this DNA virus.
用鼠γ疱疹病毒68（MHV - 68）感染BALB/c小鼠的呼吸道，可诱导区域纵隔淋巴结（MLN）中病毒特异性细胞毒性T淋巴细胞（CTL）前体（CTLp）的克隆扩增。其中一些CTLp分化成为功能完全的CTL效应细胞，在淋巴组织和肺脏的病理部位均可检测到。虽然淋巴结和脾脏终生都含有大量潜伏感染的B细胞，但病毒特异性CTL活性在所有部位均迅速下降。在呼吸道上皮中MHV - 68感染的增殖期结束后的几个月内，MLN中的CD8⁺ CTLp数量降至背景水平，但在脾脏中维持在相对较低的频率。在培养的脾细胞中也可证明存在产生γ干扰素的、MHV - 68特异性CD4⁺细胞群。病毒特异性免疫球蛋白G（IgG）反应发展缓慢，血清中和抗体和酶联免疫吸附测定滴度持续上升数月。感染后2周内血清总IgG水平急剧升高，可能是多克隆B细胞活化的结果，并保持在高水平。这种DNA病毒的持续存在显然影响了免疫反应谱。
