Reischl U, Lohmann C P
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg.
Klin Monbl Augenheilkd. 1997 Oct;211(4):227-34. doi: 10.1055/s-2008-1035127.
The polymerase chain reaction (PCR) is a relatively new molecular biology-based technique that allows the specific identification of small amounts of DNA or RNA. This technique offers significant advantages compared to conventional microbiological tests, such as microscopy or culture. Increasing numbers of immunosuppressed patients with intraocular infections and, improvements in antibiotic therapy for specific organisms require a more accurate diagnosis. PCR is capable of providing such requirements. This technique is evolving rapidly and in cooperation with clinicians and molecular biologists a number of applications are being developed, which contribute to the growing demands on microbiological testings. This article describes the basics, indications and the limitations of the polymerase chain reaction.
聚合酶链反应（PCR）是一种相对较新的基于分子生物学的技术，可用于特异性鉴定少量DNA或RNA。与传统的微生物学检测方法（如显微镜检查或培养）相比，该技术具有显著优势。越来越多的免疫抑制患者发生眼内感染，以及针对特定病原体的抗生素治疗方法不断改进，都需要更准确的诊断。PCR能够满足这些需求。该技术正在迅速发展，并且与临床医生和分子生物学家合作，正在开发许多应用，这也导致了对微生物检测的需求不断增加。本文介绍了聚合酶链反应的基本原理、适应证和局限性。
