编码半乳糖凝集素-6的小鼠Lgals6基因的序列、结构及染色体定位。

Sequence, structure, and chromosomal mapping of the mouse Lgals6 gene, encoding galectin-6.

作者信息

Gitt M A, Xia Y R, Atchison R E, Lusis A J, Barondes S H, Leffler H

机构信息

Center for Neurobiology and Psychiatry, Department of Psychiatry, University of California, San Francisco, California 94143-0984, USA.

出版信息

J Biol Chem. 1998 Jan 30;273(5):2961-70. doi: 10.1074/jbc.273.5.2961.

DOI:10.1074/jbc.273.5.2961

PMID:9446609

Abstract

In the accompanying paper (Gitt, M. A., Colnot, C., Poirier, F., and Barondes, S. H., and Leffler, H. (1998) J. Biol. Chem. 273, 2954-2960), we reported that mouse gastrointestinal tract specifically expresses two closely related galectins, galectins-4 and -6, each with two carbohydrate recognition domains in the same peptide. Here, we report the isolation, characterization, and chromosomal mapping of the complete mouse Lgals6 gene, which encodes galectin-6, and of a fragment of a distinct gene, Lgals4, which encodes galectin-4. The coding sequence of galectin-6 is specified by eight exons. The upstream region contains two putative promoters. Both Lgals6 and the closely related Lgals4 are clustered together about 3.2 centimorgans proximal to the apoE gene on mouse chromosome 7. The syntenic human region is 19q13.1-13.3.

摘要

在随附论文（吉特，M. A.，科尔诺，C.，波里耶，F.，巴伦德斯，S. H.，以及莱弗勒，H.（1998年）《生物化学杂志》273卷，2954 - 2960页）中，我们报道小鼠胃肠道特异性表达两种密切相关的半乳糖凝集素，即半乳糖凝集素 - 4和 - 6，它们在同一肽链中各有两个碳水化合物识别结构域。在此，我们报道完整的小鼠Lgals6基因（编码半乳糖凝集素 - 6）以及一个不同基因Lgals4（编码半乳糖凝集素 - 4）的片段的分离、特征分析及染色体定位。半乳糖凝集素 - 6的编码序列由八个外显子确定。上游区域包含两个假定的启动子。Lgals6和密切相关的Lgals4都聚集在小鼠7号染色体上载脂蛋白E基因近端约3.2厘摩处。人类同线性区域为19q13.1 - 13.3。