Telford E A, Wightman P, Leek J, Markham A F, Lench N J, Bonthron D T
Molecular Medicine Unit, St. James's University Hospital, University of Leeds, United Kingdom.
Biochem Biophys Res Commun. 1998 Jan 14;242(2):407-12. doi: 10.1006/bbrc.1997.7977.
We report the cloning and characterization of a novel human kinesin-like gene with strong homology to the mouse kinesin Kif3c. The full-length cDNA contains an open reading frame of 2382 nucleotides encoding a predicted 793 amino acid peptide that includes a 389 amino acid motor domain conserved among other kinesins. PCR and DNA sequence analysis of PAC clones containing the human KIF3C sequence revealed that the gene contains 8 exons. All introns have the conserved GT and AG dinucleotides present at their donor and acceptor sites, respectively. We have localized KIF3C to chromosome band 2p23 by fluorescence in situ hybridization.
我们报道了一个与小鼠驱动蛋白Kif3c具有高度同源性的新型人类驱动蛋白样基因的克隆和特性分析。全长cDNA包含一个2382个核苷酸的开放阅读框，编码一个预测的793个氨基酸的肽，其中包括一个在其他驱动蛋白中保守的389个氨基酸的运动结构域。对包含人类KIF3C序列的PAC克隆进行PCR和DNA序列分析表明，该基因含有8个外显子。所有内含子在其供体和受体位点分别具有保守的GT和AG二核苷酸。我们通过荧光原位杂交将KIF3C定位到染色体2p23带。
