Sardella M, Navone F, Rocchi M, Rubartelli A, Viggiano L, Vignali G, Consalez G G, Sitia R, Cabibbo A
DIBIT-HSR, Via Olgettina 58, Milan, 20132, Italy.
Genomics. 1998 Feb 1;47(3):405-8. doi: 10.1006/geno.1997.5123.
Kinesins are microtubule-dependent molecular motors involved in intracellular transport and mitosis. Here, we report the cloning, sequencing, mapping, and expression of a novel member of the kinesin superfamily. The sequence of this newly identified human cDNA reveals an open reading frame encoding a putative protein of 792 residues. Based on its high sequence similarity to the kinesin-like molecule KIF3B, we named this protein KIF3C. KIF3C is encoded by transcripts that are distinct from the KIF3B mRNA in human, rat, and mouse and is preferentially expressed in the brain. Fluorescence in situ hybridization reveals that, in the human genome, the KIF3C gene maps to chromosome 2 at 2p23. The sequence of KIF3C predicts an unusually long insertion in the proximity of L11, a region thought to mediate microtubule binding. Taken together, these findings suggest that KIF3C is a novel kinesin-like protein that might be specifically involved in microtubule-based transport in neuronal cells.
驱动蛋白是依赖微管的分子马达，参与细胞内运输和有丝分裂。在此，我们报告驱动蛋白超家族一个新成员的克隆、测序、定位及表达。这个新鉴定的人类cDNA序列揭示了一个开放阅读框，编码一个含792个残基的推定蛋白。基于其与驱动蛋白样分子KIF3B的高度序列相似性，我们将此蛋白命名为KIF3C。KIF3C由与人类、大鼠和小鼠中KIF3B mRNA不同的转录本编码，且在脑中优先表达。荧光原位杂交显示，在人类基因组中，KIF3C基因定位于2号染色体的2p23。KIF3C的序列预测在L11附近有一个异常长的插入，L11区域被认为介导微管结合。综上所述，这些发现表明KIF3C是一种新型的驱动蛋白样蛋白，可能特异性参与神经元细胞中基于微管的运输。
