Katz D, Ben-Moshe H, Alon S
Israel Institute for Biological Research, Ness-Ziona, Israel.
J Clin Microbiol. 1976 Mar;3(3):227-32. doi: 10.1128/jcm.3.3.227-232.1976.
Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and compared to the plaque neutralization and microhemagglutination inhibition test. Reproducibility of the microneutralization test was statistically analyzed.
使用微量滴定系统，在生长于“U”型或平底培养板中的鸡胚成纤维细胞中进行新城疫病毒感染性和中和抗体的滴定。通过联合血细胞吸附-血细胞凝集方法检测感染性。该反应的抑制表明存在中和抗体。开发了一种24小时微量中和试验，并与空斑中和试验和微量血细胞凝集抑制试验进行比较。对微量中和试验的可重复性进行了统计学分析。
