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牛胰脂肪酶。III. 油脂的脂肪分解作用及脂肪酸特异性。

Bovine pancreatic lipase. III. Lipolysis of oils and fats and fatty acid specificity.

作者信息

Chandan R C, Khan I M, Shahani K M

出版信息

J Dairy Sci. 1976 May;59(5):847-53. doi: 10.3168/jds.S0022-0302(76)84286-5.

DOI:10.3168/jds.S0022-0302(76)84286-5
PMID:944720
Abstract

Purified bovine pancreatic lipase hydrolyzed butteroil, vegetable oils, and synthetic glycerides. The enzyme hydrolyzed triglycerides more rapidly than di- and monoglycerides and tripropionin faster than any other synthetic glyceride. Triacetin was the least hydrolyzed glyceride. Gas-liquid chromatographic analysis of the free fatty acids liberated by the lipase from milk fat indicated that the enzyme selectively liberated butyric acid in higher proportion than the relative amount originally in the fat. The enzyme released saturated as well as unsaturated fatty acids from commericial vegetable oils. With regard to the lipolytic behavior, in general, the bovine pancreatic lipase closely resembled milk lipase.

摘要

纯化的牛胰脂肪酶可水解乳脂、植物油和合成甘油酯。该酶水解甘油三酯的速度比甘油二酯和甘油单酯更快,水解三丙酸甘油酯的速度比任何其他合成甘油酯都快。三醋精是水解最少的甘油酯。对脂肪酶从乳脂中释放出的游离脂肪酸进行气液色谱分析表明,该酶选择性释放的丁酸比例高于脂肪中原本的相对含量。该酶还从商业植物油中释放出饱和脂肪酸和不饱和脂肪酸。总体而言,就脂解行为而言,牛胰脂肪酶与乳脂肪酶非常相似。

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