Kerr D E, Liang F, Bondioli K R, Zhao H, Kreibich G, Wall R J, Sun T T
Gene Evaluation and Mapping Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Nat Biotechnol. 1998 Jan;16(1):75-9. doi: 10.1038/nbt0198-75.
Uroplakin genes are expressed in a bladder-specific and differentiation-dependent fashion. Using a 3.6-kb promoter of mouse uroplakin II gene, we have generated transgenic mice that express human growth hormone (hGH) in their bladder epithelium, resulting in its secretion into the urine at 100-500 ng/ml. The levels of urine hGH concentration remain constant for longer than 8 months. hGH is present as aggregates mostly in the uroplakin-delivering cytoplasmic vesicles that are targeted to fuse with the apical surface. Using the bladder as a bioreactor offers unique advantages, including the utility of all animals throughout their lives. Using urine, which contains little protein and lipid, as a starting material facilitates recombinant protein purification.
uroplakin基因以膀胱特异性和分化依赖性方式表达。利用小鼠uroplakin II基因的3.6 kb启动子，我们培育出了在膀胱上皮中表达人生长激素（hGH）的转基因小鼠，导致其以100 - 500 ng/ml的浓度分泌到尿液中。尿液hGH浓度水平在超过8个月的时间里保持恒定。hGH主要以聚集体形式存在于靶向与顶端表面融合的uroplakin运输细胞质囊泡中。将膀胱用作生物反应器具有独特优势，包括所有动物一生都可利用。以几乎不含蛋白质和脂质的尿液作为起始材料便于重组蛋白的纯化。
