Mou C, Ganju N, Sridhar K S, Krishan A
Department of Radiation Oncology and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, FL 33136, USA.
J Chromatogr B Biomed Sci Appl. 1997 Dec 5;703(1-2):217-24. doi: 10.1016/s0378-4347(97)00414-3.
A high-performance liquid chromatographic method has been developed and tested for simultaneous extraction, elution and determination of doxorubicin and prochlorperazine content in human plasma samples. The procedure consists of extraction through a conditioned C18 solid-phase extraction cartridge, elution from a Spherisorb C8 reversed-phase column by an isocratic mobile phase (60% acetonitrile, 15% methanol and 25% buffer) followed by detection with electrochemical and fluorescence detectors. Recovery of doxorubicin and prochlorperazine from pooled human plasma samples (n=3) containing 100 ng/ml of the two drugs was 77.8+/-3.5% and 89.1+/-6.0%, respectively. The lower limits of quantitation for doxorubicin and prochlorperazine in plasma samples were 6.25 ng/ml and 10 ng/ml, respectively. A linear calibration curve was obtained for up to 2 microg/ml of doxorubicin and prochlorperazine. This combination method may be of particular value in clinical studies where phenothiazines such as prochlorperazine are used to enhance retention of doxorubicin in drug resistant tumor cells.
已开发并测试了一种高效液相色谱法，用于同时提取、洗脱和测定人血浆样品中阿霉素和氯丙嗪的含量。该方法包括通过预处理的C18固相萃取柱进行萃取，用等度流动相（60%乙腈、15%甲醇和25%缓冲液）从Spherisorb C8反相柱上洗脱，然后用电化学和荧光检测器进行检测。从含有100 ng/ml这两种药物的混合人血浆样品（n=3）中回收阿霉素和氯丙嗪的回收率分别为77.8±3.5%和89.1±6.0%。血浆样品中阿霉素和氯丙嗪的定量下限分别为6.25 ng/ml和10 ng/ml。对于高达2 μg/ml的阿霉素和氯丙嗪，获得了线性校准曲线。这种联合方法在临床研究中可能具有特殊价值，在这些研究中，如氯丙嗪等吩噻嗪类药物被用于增强阿霉素在耐药肿瘤细胞中的保留。
