Cooper J K, McKay G, Midha K K
J Pharm Sci. 1983 Nov;72(11):1259-62. doi: 10.1002/jps.2600721106.
A specific and sensitive high-performance liquid chromatographic (HPLC) method for the quantitative determination of subnanogram levels of chlorpromazine in plasma is described. Following extraction of chlorpromazine and the internal standard, prochlorperazine, HPLC analysis is carried out on a cyano column with a mobile phase consisting of 0.1 M ammonium acetate in acetonitrile (10:90 v/v). The use of oxidative thin-layer amperometric detection allowed the quantitation of 0.25 ng of chlorpromazine/ml of plasma with a coefficient of variation of 5.1%. The HPLC method has adequate sensitivity to follow plasma concentration-time profiles up to 24 hr following low single oral doses of chlorpromazine in healthy volunteers.
本文描述了一种用于定量测定血浆中亚纳克级氯丙嗪的特异性灵敏高效液相色谱（HPLC）方法。在提取氯丙嗪和内标物丙氯拉嗪后，采用氰基柱进行HPLC分析，流动相为乙腈中0.1M醋酸铵（10:90 v/v）。使用氧化薄层安培检测法可定量测定血浆中0.25 ng/ml的氯丙嗪，变异系数为5.1%。该HPLC方法具有足够的灵敏度，可跟踪健康志愿者单次口服低剂量氯丙嗪后长达24小时的血浆浓度-时间曲线。
