胰高血糖素对运动大鼠肝糖原动员的影响。

The influence of glucagon on hepatic glycogen mobilization in exercising rats.

作者信息

Galbo H, Holst J J

出版信息

Pflugers Arch. 1976 May 6;363(1):49-53. doi: 10.1007/BF00587401.

Abstract

The significance of glucagon for the alterations in carbohydrate and fat metabolism during swimming has been evaluated. Fed, male rats were used. Blood was drawn by cardiac puncture for glucose analysis and either rabbit-antiglucagonserum (A-rats) or normal rabbitserum (N-rats) injected. Twenty-nine rats were then forced to swim (S-rats) with a tail weight for 60 min, while 16 rats were resting controls (C-rats). Subsequently blood was drawn and samples of liver and muscle tissue collected. In SN-rats glucagon concentrations increased from 152 +/- 18 (S.E.) pg/ml (CN-rats) to 332 +/- 61 (P less than 0.05), while liver glycogen decreased (P less than 0.001) and blood glucose increased (P less than 0.05). In SA-rats, however, the changes in liver glycogen and blood glucose were halved indicating that increased glucagon secretion enhances hepatic glycogen depletion during prolonged exercise. NEFA rose in SA-rats (P less than 0.005) as well as in SN-rats (P less than 0.05). Glycerol concentrations, however, only increased in SA-rats (P less than 0.05) indicating a shift towards lipid combustion in antibody treated rats. Muscle glycogen and plasma insulin diminished and blood lactate increased uniformly in exercised rats.

摘要

已对胰高血糖素在游泳过程中碳水化合物和脂肪代谢变化中的作用进行了评估。使用的是喂食后的雄性大鼠。通过心脏穿刺采血进行葡萄糖分析，并注射兔抗胰高血糖素血清（A组大鼠）或正常兔血清（N组大鼠）。然后，29只大鼠负重尾巴被迫游泳60分钟（S组大鼠），而16只大鼠作为静息对照组（C组大鼠）。随后采血，并采集肝脏和肌肉组织样本。在SN组大鼠中，胰高血糖素浓度从152±18（标准误）pg/ml（CN组大鼠）增至332±61（P<0.05），而肝糖原减少（P<0.001），血糖升高（P<0.05）。然而，在SA组大鼠中，肝糖原和血糖的变化减半，这表明胰高血糖素分泌增加会增强长时间运动期间肝脏糖原的消耗。SA组大鼠（P<0.005）和SN组大鼠（P<0.05）的非酯化脂肪酸均升高。然而，甘油浓度仅在SA组大鼠中升高（P<0.05），这表明在接受抗体处理的大鼠中，代谢向脂质燃烧转变。运动大鼠的肌肉糖原和血浆胰岛素减少，血乳酸均一性增加。