通过实时代谢监测测定大肠杆菌中的抗生素后效应。
Postantibiotic effect in Escherichia coli determined with real-time metabolic monitoring.
作者信息
Libby J M
机构信息
Molecular Devices Corporation, Sunnyvale, California 94089, USA.
出版信息
Antimicrob Agents Chemother. 1998 Jan;42(1):78-82. doi: 10.1128/AAC.42.1.78.
Abstract
Metabolic activity was used to quantify the delay in recovery of Escherichia coli after limited exposures to cefotaxime and piperacillin. This was measured with a microphysiometer, a device which measures changes in pH in the cellular environment, which in turn reflects the metabolic activity of the cells. The antibiotics were rapidly pumped into and flushed from each cellular environment. The length of time that the bacteria were exposed to either antibiotic was determined by programmed valve changes. Metabolic activity was measured during periods in which the instrument's pumps were off. Graphical analysis of the data was used to determine the postantibiotic effect. The lengths of the postantibiotic effects of both drugs (95 to 101 min) determined with the microphysiometer corresponded with the reappearance of short, highly motile cells in significant numbers.
摘要
通过代谢活性来量化大肠杆菌在有限接触头孢噻肟和哌拉西林后恢复的延迟情况。这是用微生理仪进行测量的，该仪器可测量细胞环境中的pH变化，而这又反映了细胞的代谢活性。抗生素被快速泵入每个细胞环境并冲洗出来。细菌接触每种抗生素的时间长度由程序控制的阀门变化来确定。在仪器泵关闭期间测量代谢活性。通过对数据进行图形分析来确定抗生素后效应。用微生理仪测定的两种药物的抗生素后效应时长（95至101分钟）与大量短而高度活跃的细胞重新出现相对应。
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