Dondua A K, Kostyuchenko R P, Fedorova Z E
Department of Embryology, St. Petersburg State University, Russia.
Int J Dev Biol. 1997 Dec;41(6):853-8.
The ooplasmic segregation in the Nereis virens egg starts after completion of cortical reaction. During ooplasmic segregation the radial symmetrical pattern of the oocyte transforms into polarized stratified structure with different kinds of cytoplasm regularly distributed along the animal-vegetal axis. The beginning of oocyte polarization is associated with the generation of the mitotic spindle and is sensitive to microtubule inhibitors. By analyzing the effect of colchicine, taxol, nocodazole and cytochalasin B, we show that the mechanisms of ooplasmic segregation have an integrated complex nature and include interactions of microtubules and microfilaments. The inhibition of any of these elements delays or disturbs the ooplasmic segregation and simultaneous disruption of microtubules and microfilaments functions leads to total cessation of the process.
沙蚕卵中的卵质分离在皮层反应完成后开始。在卵质分离过程中，卵母细胞的辐射对称模式转变为极化分层结构，不同类型的细胞质沿动物 - 植物轴规则分布。卵母细胞极化的开始与有丝分裂纺锤体的产生相关，并且对微管抑制剂敏感。通过分析秋水仙碱、紫杉醇、诺考达唑和细胞松弛素B的作用，我们表明卵质分离机制具有综合复杂的性质，包括微管和微丝的相互作用。对这些元素中任何一个的抑制都会延迟或干扰卵质分离，而微管和微丝功能的同时破坏会导致该过程完全停止。
