Farooq S, Bailey E, Farmer P B, Jukes R, Lamb J H, Hernández H, Sram R, Topinka J
M.R.C. Toxicology Unit, University of Leicester, UK.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):49-60. doi: 10.1016/s0378-4347(97)00364-2.
A novel method for the determination of cis-thymine glycol in DNA has been developed, using gas chromatography-mass spectrometry with selected ion recording or multiple reaction monitoring. The procedure involves acidic hydrolysis of DNA in the presence of the internal standard cis-[2H3]thymine glycol, followed by derivatisation with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide. The method was validated on DNA that had been oxidatively modified in vitro by radiation treatment, and was then applied to determine cis-thymine glycol in human placental DNA. Background levels of 5.45+/-2.98 ng cis-thymine glycol/mg DNA were observed in the human samples.
已开发出一种测定DNA中顺式胸腺嘧啶二醇的新方法，该方法采用带选择离子记录或多反应监测的气相色谱-质谱联用技术。该程序包括在内部标准顺式-[2H3]胸腺嘧啶二醇存在下对DNA进行酸性水解，然后用N-甲基-N-(叔丁基二甲基甲硅烷基)三氟乙酰胺进行衍生化。该方法在经辐射处理体外氧化修饰的DNA上进行了验证，然后用于测定人胎盘DNA中的顺式胸腺嘧啶二醇。在人体样本中观察到顺式胸腺嘧啶二醇的背景水平为5.45±2.98 ng/mg DNA。
