Cox S K, Orosz S, Burnette J, Frazier D
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):175-80. doi: 10.1016/s0378-4347(97)00378-2.
A simple, rapid and sensitive method for the extraction and HPLC analysis of itraconazole and hydroxyitraconazole in tissue and plasma or serum is described. Tissue (5-100 mg) and plasma (0.1 ml) underwent a simple extraction into methanol. Chromatography was performed on a Novapak C18 column using a mobile phase of water-acetonitrile-diethylamine (42:58:0.05, v/v), pH 2.45, with a flow-rate of 1.5 ml/min. Fluorescence was measured at excitation 260 nm and emission 365 nm. The procedure produced a linear curve for the concentration range 10-1000 ng/ml. The development of the assay produced accurate, rapid repeatable results for both tissue and plasma or serum.
本文描述了一种用于组织、血浆或血清中伊曲康唑和羟基伊曲康唑的提取及高效液相色谱分析的简单、快速且灵敏的方法。组织（5 - 100毫克）和血浆（0.1毫升）用甲醇进行简单提取。在诺瓦帕克C18柱上进行色谱分析，流动相为水 - 乙腈 - 二乙胺（42:58:0.05，v/v），pH 2.45，流速为1.5毫升/分钟。在激发波长260纳米和发射波长365纳米处测量荧光。该方法在10 - 1000纳克/毫升的浓度范围内产生线性曲线。该分析方法对组织、血浆或血清均能产生准确、快速且可重复的结果。
