血浆和组织活检中阿霉素的测定。

Determination of adriamycin in plasma and tissue biopsies.

作者信息

Cox S K, Wilke A V, Frazier D

机构信息

Department of Environmental Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.

出版信息

J Chromatogr. 1991 Mar 8;564(1):322-9. doi: 10.1016/0378-4347(91)80098-w.

DOI:10.1016/0378-4347(91)80098-w

PMID:1860929

Abstract

A simple, rapid and sensitive method for the extraction and high-performance liquid chromatographic analysis of adriamycin in tissue and plasma is described. Tissue (5-100 mg) and plasma (1 ml) samples underwent a C18 Sep-Pak extraction into methanol. Chromatography was performed on a muBondapakphenyl column using a mobile phase of acetonitrile-0.1 M ammonium formate (pH 4.0) with a flow-rate of 2 ml/min. Fluorometric detection was used with an excitation of 480 nm and an emission of 550 nm. The procedure produced a linear curve for the concentration range 25-1000 ng/ml. The development of the assay produced rapid, repeatable and accurate results for both small tissue samples and plasma.

摘要

本文描述了一种用于组织和血浆中阿霉素提取及高效液相色谱分析的简单、快速且灵敏的方法。组织（5 - 100毫克）和血浆（1毫升）样品用甲醇通过C18 Sep - Pak柱进行提取。色谱分析在μBondapak苯基柱上进行，流动相为乙腈 - 0.1 M甲酸铵（pH 4.0），流速为2毫升/分钟。采用荧光检测，激发波长为480纳米，发射波长为550纳米。该方法在25 - 1000纳克/毫升的浓度范围内产生线性曲线。该检测方法对于小组织样品和血浆均能产生快速、可重复且准确的结果。