用于定量测定人血浆中长春西汀的改进气相色谱 - 质谱法。
Improved gas chromatographic-mass spectrometric method for the quantitative determination of vinpocetine in human plasma.
作者信息
Vatsova M, Tzvetanov S, Drenska A, Goranscheva J, Tyutyulkova N
机构信息
Chemical Pharmaceutical Research Institute, Sofia, Bulgaria.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):221-6. doi: 10.1016/s0378-4347(97)00365-4.
An improved and validated method for the determination of vinpocetine in human plasma using a combination of a solid-phase extraction as a cleaning step followed by gas chromatography-mass spectrometry (GC-MS) has been presented. Quantitation has been carried out with apovincaminic acid methyl ester as internal standard. A limit of detection of 0.01 ng/ml, limit of quantitation of 0.10 ng/ml, as well as a satisfactory accuracy, improved precision and prolonged capillary column life have been achieved.
本文提出了一种改进且经过验证的方法，用于测定人血浆中的长春西汀。该方法采用固相萃取作为净化步骤，随后结合气相色谱 - 质谱联用（GC-MS）。采用阿朴长春胺酸甲酯作为内标进行定量分析。实现了0.01 ng/ml的检测限、0.10 ng/ml的定量限，以及令人满意的准确度、更高的精密度和延长的毛细管柱寿命。
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