Faure J D, Vittorioso P, Santoni V, Fraisier V, Prinsen E, Barlier I, Van Onckelen H, Caboche M, Bellini C
Laboratoire de Biologie Cellulaire, Versailles, France.
Development. 1998 Mar;125(5):909-18. doi: 10.1242/dev.125.5.909.
The control of cell division by growth regulators is critical to proper plant development. The isolation of single-gene mutants altered in the response to plant hormones should permit the identification of essential genes controlling the growth and development of plants. We have isolated mutants pasticcino belonging to 3 complementation groups (pas1, pas2, pas3) in the progeny of independent ethyl methane sulfonate and T-DNA mutagenized Arabidopsis thaliana plants. The screen was performed in the presence or absence of cytokinin. The mutants isolated were those that showed a significant hypertrophy of their apical parts when grown on cytokinin-containing medium. The pas mutants have altered embryo, leaf and root development. They display uncoordinated cell divisions which are enhanced by cytokinin. Physiological and biochemical analyses show that cytokinins are probably involved in pas phenotypes. The PAS genes have been mapped respectively to chromosomes 3, 5 and 1 and represent new plant genes involved in the control of cell division and plant development.
生长调节剂对细胞分裂的控制对于植物的正常发育至关重要。分离出对植物激素反应发生改变的单基因突变体应有助于鉴定控制植物生长和发育的必需基因。我们在独立的甲磺酸乙酯和T-DNA诱变的拟南芥植株后代中分离出了属于3个互补群（pas1、pas2、pas3）的pasticcino突变体。筛选是在有或没有细胞分裂素的情况下进行的。分离出的突变体是那些在含细胞分裂素的培养基上生长时顶端部分出现明显肥大的突变体。pas突变体的胚胎、叶片和根系发育发生了改变。它们表现出不协调的细胞分裂，这种分裂会被细胞分裂素增强。生理和生化分析表明，细胞分裂素可能与pas表型有关。PAS基因已分别定位到第3、5和1号染色体上，代表参与细胞分裂和植物发育控制的新植物基因。
