爬行动物降钙素的核苷酸序列：它们与鸡降钙素的高度同源性。

Nucleotide sequences of reptile calcitonins: their high homology to chicken calcitonin.

作者信息

Suzuki N, Eguchi C, Hirai T, Sasayama Y

机构信息

Noto Marine Laboratory, Faculty of Science, Kanazawa University, Ishikawa, Japan.

出版信息

Zoolog Sci. 1997 Oct;14(5):833-6. doi: 10.2108/zsj.14.833.

DOI:10.2108/zsj.14.833

PMID:9450396

Abstract

The calcitonin genes of four species of reptiles (Reeve's turtle, rat snake, grass lizard, and spectacled caiman) were amplified from the genomic DNA, as well as from the mRNA of the ultimobranchial glands of the former three species, by the polymerase chain reaction (PCR) method, and were sequenced. Among several primer sets, only one primer set synthesized from the chicken calcitonin gene was compatible with those of the reptiles. The nucleotide sequences of the reptile calcitonin genes were highly homologous with that of chicken calcitonin (100% for turtle, 99% for caiman, 96% for lizard and 93% for snake). The products amplified from mRNA by the RT-PCR method matched completely those from genomic DNA in the turtle, snake and lizard.

摘要

采用聚合酶链反应（PCR）方法，从四种爬行动物（乌龟、锦蛇、草蜥和眼镜凯门鳄）的基因组DNA以及前三种动物终末鳃腺的mRNA中扩增出降钙素基因，并进行测序。在几组引物中，只有一组由鸡降钙素基因合成的引物与爬行动物的引物兼容。爬行动物降钙素基因的核苷酸序列与鸡降钙素的核苷酸序列高度同源（乌龟为100%，凯门鳄为99%，蜥蜴为96%，蛇为93%）。通过逆转录聚合酶链反应（RT-PCR）方法从mRNA扩增出的产物与乌龟、蛇和蜥蜴基因组DNA扩增出的产物完全匹配。