鸡胚心脏质膜流动性的发育变化

Developmental changes in plasma membrane fluidity in chick embryo heart.

Kutchai H, Barenholz Y, Ross T F, Wermer D E

Biochim Biophys Acta. 1976 Jun 4;436(1):101-12. doi: 10.1016/0005-2736(76)90223-6.

Abstract

Decreases in the rate of transport of sugars (facilitated transport), amino acids (active transport), and urea (simple diffusion) occur in chick embryo heart during development. This work considers the possibility that changes in the plasma membrane fluidity during development contribute to the observed changes in transport activities. 2. Technics were developed for subcellar fractionation of chick embryos and adult chickens. 3. The depolarization of the fluorescence of 1,6-diphenylhexatriene was used to estimate the fluidity of the lipid portion of plasma membrane enriched fractions of hearts from chick embryos at various stages of development and from adult hearts. 4. There is a pattern of decreasing membrane viscosity as development proceeds. Between 5-6 days and 10 days of embryonic life a 20% decrease in viscosity of the plasma membrane-enriched fraction occurs. Between 10 and 20 days of embryonic life there is no significant change in viscosity. Between 20 days of development (1 day before hatching) and adulthood there is a further 55% decrease in plasma membrane viscosity. 5. It is proposed that the changes in membrane fluidity observed may contribute to developmental changes in membrane transport activities, but other factors must also be involved.

摘要

在鸡胚心脏发育过程中，糖类（易化转运）、氨基酸（主动转运）和尿素（简单扩散）的转运速率会降低。本研究探讨了发育过程中质膜流动性的变化是否导致了所观察到的转运活性变化。2. 开发了用于鸡胚和成年鸡亚细胞分级分离的技术。3. 利用1,6 - 二苯基己三烯荧光的去极化来估计不同发育阶段鸡胚心脏以及成年鸡心脏富含质膜部分的脂质流动性。4. 随着发育进行，存在膜粘度降低的模式。在胚胎期5 - 6天至10天之间，富含质膜部分的粘度降低20%。在胚胎期10天至20天之间，粘度无显著变化。在发育20天（孵化前1天）至成年期之间，质膜粘度进一步降低55%。5. 有人提出，观察到的膜流动性变化可能有助于膜转运活性的发育变化，但也必定涉及其他因素。