鸡胚发育期间及出生后心室质膜的流动性：自旋标记和荧光研究。
Fluidity of chicken ventricular plasma membranes during development in-ovo and after birth: spin labelling and fluorescence studies.
作者信息
Daveloose D, Vezin H, Basse F, Viret J
机构信息
CRSSA, unité de Biophysique, La Tronche, France.
出版信息
J Mol Cell Cardiol. 1993 Dec;25(12):1439-44. doi: 10.1006/jmcc.1993.1160.
The fluidity of isolated chicken ventricular plasma membranes was investigated by ESR spectroscopy, using three doxylstearic spin labels (5-SASL, 10-SASL and 16-SASL), and by fluorescence anisotropy measurements, using DPH probe. The analysis of 2T1 splitting constant and rotational correlation frequency have shown differences, during in-ovo development, characterized by a rigidification phase with a maximum at 19 days of development, just before hatching, followed by a fluidification phase after birth. These phenomena were also improved by DPH fluorescence and dynamical analysis of the hydrophobic 16-SASL probe.
利用电子自旋共振光谱法，使用三种硬脂酰基氮氧自由基自旋标记物（5-硬脂酰基氮氧自由基、10-硬脂酰基氮氧自由基和16-硬脂酰基氮氧自由基），以及荧光各向异性测量法，使用二苯基己三烯（DPH）探针，对分离出的鸡心室质膜的流动性进行了研究。对2T1分裂常数和旋转相关频率的分析表明，在胚胎发育过程中存在差异，其特征是在孵化前19天发育至最大程度时出现僵化阶段，随后在出生后进入流化阶段。这些现象也通过DPH荧光以及疏水性16-硬脂酰基氮氧自由基探针的动力学分析得到了证实。
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