Brodie H A, Thompson T C, Vassilian L, Lee B N
Department of Otolaryngology, University of California, Davis, School of Medicine, USA.
Otolaryngol Head Neck Surg. 1998 Jan;118(1):15-21. doi: 10.1016/S0194-5998(98)70369-9.
Newly formed disorganized bone fills the open spaces within the otic capsule in various pathologic conditions, resulting in labyrinthitis ossificans. The pathologic mechanisms of this disease remain poorly understood. To better study the sequence of events and contributing mechanisms involved in labyrinthitis ossificans, an animal model was developed. Three groups of Mongolian gerbils received either an intralabyrinthine injection of normal saline solution (group 1) or Streptococcus pneumoniae polysaccharide capsule antigens (groups 2 and 3). The temporal bones were harvested after 3 months and serially sectioned. None of the eight control animals (group 1), which received intralabyrinthine injections of normal saline solution had any histologic changes in their temporal bones. Nine of the surviving 19 animals in groups 2 and 3 had fibrosis or evidence of early ossification. A fourth group of Mongolian gerbils received two intrathecal injections of live S. pneumoniae organisms. The temporal bones were harvested after 3 months and serially sectioned. Fourteen of the surviving 15 animals had fibrosis or ossification or both. This animal model will provide a method for study of the mechanisms of labyrinthitis ossificans.
在各种病理情况下，新形成的紊乱骨填充了耳囊内的开放空间，导致骨化性迷路炎。该疾病的病理机制仍知之甚少。为了更好地研究骨化性迷路炎所涉及的事件顺序和促成机制，建立了一种动物模型。三组蒙古沙鼠分别接受迷路内注射生理盐水（第1组）或肺炎链球菌多糖荚膜抗原（第2组和第3组）。3个月后取出颞骨并进行连续切片。接受迷路内注射生理盐水的8只对照动物（第1组）的颞骨均无组织学变化。第2组和第3组存活的19只动物中有9只出现纤维化或早期骨化迹象。第四组蒙古沙鼠接受两次鞘内注射活的肺炎链球菌。3个月后取出颞骨并进行连续切片。存活的15只动物中有14只出现纤维化或骨化或两者皆有。这种动物模型将为研究骨化性迷路炎的机制提供一种方法。
