目标1：一种胞质分裂所需的哺乳动物中体相关蛋白。
AIM-1: a mammalian midbody-associated protein required for cytokinesis.
作者信息
Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M
机构信息
Louis Pasteur Center for Medical Research, Department of Molecular Biology, 103-5 Tanaka Monzencho, Sakyo-ku, Kyoto 606.
出版信息
EMBO J. 1998 Feb 2;17(3):667-76. doi: 10.1093/emboj/17.3.667.
Abstract
Mitosis is a highly coordinated process that assures the fidelity of chromosome segregation. Errors in this process result in aneuploidy which can lead to cell death or oncogenesis. In this paper we describe a putative mammalian protein kinase, AIM-1 (Aurora and Ipl1-like midbody-associated protein), related to Drosophila Aurora and Saccharomyces cerevisiae Ipl1, both of which are required for chromosome segregation. AIM-1 message and protein accumulate at G2/M phase. The protein localizes at the equator of central spindles during late anaphase and at the midbody during telophase and cytokinesis. Overexpression of kinase-inactive AIM-1 disrupts cleavage furrow formation without affecting nuclear division. Furthermore, cytokinesis frequently fails, resulting in cell polyploidy and subsequent cell death. These results strongly suggest that AIM-1 is required for proper progression of cytokinesis in mammalian cells.
摘要
有丝分裂是一个高度协调的过程，可确保染色体分离的准确性。该过程中的错误会导致非整倍体，进而可能导致细胞死亡或肿瘤发生。在本文中，我们描述了一种假定的哺乳动物蛋白激酶AIM-1（极光激酶和Ipl1样中体相关蛋白），它与果蝇极光激酶和酿酒酵母Ipl1相关，这两种蛋白都是染色体分离所必需的。AIM-1的信使核糖核酸和蛋白质在G2/M期积累。该蛋白在后期末期定位于中央纺锤体的赤道，在末期和胞质分裂期间定位于中体。激酶失活的AIM-1的过表达会破坏分裂沟的形成，而不影响核分裂。此外，胞质分裂经常失败，导致细胞多倍体和随后的细胞死亡。这些结果强烈表明，AIM-1是哺乳动物细胞中胞质分裂正常进行所必需的。
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