Lidman K, Biberfeld G, Fagraeus A, Norberg R, Torstensson R, Utter G, Carlsson L, Luca J, Lindberg U
Clin Exp Immunol. 1976 May;24(2):266-72.
Thirty sera reacting by IFL technique in titres greater than or equal to 100 with smooth muscle fibres of rat stomach, rat renal glomeruli, and with the membrane region of thyroid cells were randomly chosen among sera sent in for routine testing of tissue antibodies. All sera but one were found to be derived from patients with chronic active hepatitis. The smooth muscle and other relevant cell staining were abolished after absorption of sera with actin, prepared from rabbit skeletal muscle and found to be homogeneous by SDS gel-electrophoresis and by electron microscopy. The actin anti-bodies were purified by precipitation of sera with F-actin and elution of the precipitates at acid pH. The purified antibodies stained all tissues in the same way as the original sera. In double immunodiffusion tests all thirty sera gave precipitation with actin. Thus, it was concluded that these broad-reacting SMA are directed against actin. The finding of high-titred SMA is of diagnostic value and supports the clinical diagnosis of active chronic hepatitis. In addition, anti-actin antibodies eluted from human sera are a suitable tool for studying actin-containing cellular structures.
在送去进行组织抗体常规检测的血清中，随机选取了30份血清，这些血清通过间接荧光抗体技术（IFL）与大鼠胃平滑肌纤维、大鼠肾小球以及甲状腺细胞的膜区域发生反应，滴度大于或等于100。除一份血清外，其余所有血清均来自慢性活动性肝炎患者。用从兔骨骼肌制备的肌动蛋白吸收血清后，平滑肌和其他相关细胞染色消失，经SDS凝胶电泳和电子显微镜检测，该肌动蛋白是均质的。通过用F -肌动蛋白沉淀血清并在酸性pH下洗脱沉淀物来纯化肌动蛋白抗体。纯化后的抗体与原始血清一样对所有组织进行染色。在双向免疫扩散试验中，所有30份血清都与肌动蛋白发生沉淀反应。因此，得出结论，这些广泛反应的平滑肌抗体（SMA）是针对肌动蛋白的。高滴度SMA的发现具有诊断价值，支持活动性慢性肝炎的临床诊断。此外，从人血清中洗脱的抗肌动蛋白抗体是研究含肌动蛋白细胞结构的合适工具。
