上皮细胞系产生粒细胞-巨噬细胞集落刺激因子：紫外线A的上调作用

GM-CSF production by epithelial cell line: upregulation by ultraviolet A.

作者信息

Park K C, Jung H C, Hwang J H, Youn S W, Ahn J S, Park S B, Kim K H, Chung J H, Youn J I

机构信息

Department of Dermatology, Seoul National University College of Medicine, Korea.

出版信息

Photodermatol Photoimmunol Photomed. 1997 Aug;13(4):133-8. doi: 10.1111/j.1600-0781.1997.tb00216.x.

DOI:10.1111/j.1600-0781.1997.tb00216.x

PMID:9453081

Abstract

It was demonstrated that UVB increases synthesis and expression of IL-1 alpha and GM-CSF by keratinocytes. Upregulation of GM-CSF by UVB is reported to be mediated by IL-1 alpha. However, regulation of IL-1 alpha and GM-CSF by UVA is not well-known. The purpose of the present study was to evaluate the effects of UVA on IL-1 alpha and GM-CSF production. Here we used a competitive RT-PCR for measuring cytokine gene expression in an epidermal cell line after UVA irradiation. IL-1 alpha and GM-CSF mRNA did not show any change at 1 h and 6 h following exposure to UVA. After UVA irradiation, however, IL-1 alpha mRNA decreased and GM-CSF mRNA increased at 24 h and the level of GM-CSF in culture supernatant increased at 24 h and 48 h. Addition of antihuman IL-1 alpha neutralizing antibody to UVA irradiated cells did not prevent the increase of GM-CSF mRNA expression. These results suggest that UVA radiation may induce GM-CSF production through an IL-1 alpha independent pathway.

摘要

已证明紫外线B（UVB）可增加角质形成细胞中白细胞介素-1α（IL-1α）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的合成与表达。据报道，UVB对GM-CSF的上调作用是由IL-1α介导的。然而，紫外线A（UVA）对IL-1α和GM-CSF的调节作用尚不明确。本研究的目的是评估UVA对IL-1α和GM-CSF产生的影响。在此，我们使用竞争性逆转录聚合酶链反应（RT-PCR）来测量UVA照射后表皮细胞系中细胞因子基因的表达。暴露于UVA后1小时和6小时，IL-1α和GM-CSF信使核糖核酸（mRNA）未显示任何变化。然而，UVA照射后，24小时时IL-1α mRNA减少，GM-CSF mRNA增加，并且培养上清液中GM-CSF的水平在24小时和48小时时升高。向UVA照射的细胞中添加抗人IL-1α中和抗体并不能阻止GM-CSF mRNA表达的增加。这些结果表明，UVA辐射可能通过一条不依赖IL-1α的途径诱导GM-CSF的产生。