Corsini E, Sangha N, Feldman S R
Department of Dermatology and Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston Salem, NC 27157-1071, USA.
Photodermatol Photoimmunol Photomed. 1997 Aug;13(4):147-52. doi: 10.1111/j.1600-0781.1997.tb00219.x.
Ultraviolet (UV) radiation induces cytokine release from cultured keratinocytes as well as from epidermis in vivo. The purpose of this study was to determine whether differentiation of cultured keratinocytes into stratified epithelium decreases the effects of UVA and UVB radiation on cytokine release. Interleukin-1 (IL-1) alpha, IL-1 beta and tumor necrosis factor (TNF)-alpha release from human keratinocytes and reconstituted human epidermis was measured after exposure to UVA or UVB radiation. Release of IL-1 alpha, IL-1 beta, and TNF-alpha was induced by both UVA and UVB radiation from both keratinocytes and reconstituted epidermis. Release of these cytokines was correlated with cytotoxicity. Keratinocyte cultures were far more sensitive to UVB radiation than reconstituted epidermis, in terms of both cytotoxicity and cytokine release. In contrast, epidermal stratification/differentiation had much less effect on the sensitivity to UVA radiation. We conclude that epidermal stratification and the formation of a stratum corneum provide protection against UVB radiation but have limited barrier effect against UVA radiation.
紫外线 (UV) 辐射可诱导培养的角质形成细胞以及体内表皮释放细胞因子。本研究的目的是确定培养的角质形成细胞分化为复层上皮是否会降低UVA和UVB辐射对细胞因子释放的影响。在暴露于UVA或UVB辐射后，测量人角质形成细胞和重组人表皮中白细胞介素-1 (IL-1)α、IL-1β和肿瘤坏死因子 (TNF)-α的释放。UVA和UVB辐射均可诱导角质形成细胞和重组表皮释放IL-1α、IL-1β和TNF-α。这些细胞因子的释放与细胞毒性相关。就细胞毒性和细胞因子释放而言，角质形成细胞培养物对UVB辐射的敏感性远高于重组表皮。相比之下，表皮分层/分化对UVA辐射敏感性的影响要小得多。我们得出结论，表皮分层和角质层的形成可提供针对UVB辐射的保护，但对UVA辐射的屏障作用有限。
