抗脂质A单克隆抗体centoxin（HA-1A）可与多种疏水性配体结合。
Anti-lipid A monoclonal antibody centoxin (HA-1A) binds to a wide variety of hydrophobic ligands.
作者信息
Helmerhorst E J, Maaskant J J, Appelmelk B J
机构信息
Department of Oral Biochemistry, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
Infect Immun. 1998 Feb;66(2):870-3. doi: 10.1128/IAI.66.2.870-873.1998.
Abstract
This note describes the binding specificities of four lipid A monoclonal antibodies (MAbs) including Centoxin (HA-1A); these MAbs display similar binding properties. MAbs reacted with lipid A and heat-killed smooth bacteria, whereas no reactivity was observed with smooth lipopolysaccharide (LPS). Immunoblotting of bacterial extracts separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the MAbs bound to many polypeptide bands including the molecular weight markers. Denaturation of bovine serum albumin (BSA) by boiling or dithiothreitol treatment unmasked antibody epitopes. In addition, binding both to a hydrophobic aliphatic C12 chain covalently coupled to BSA and to single-stranded DNA was observed. The polyreactivity of these clones is most likely mediated by a preferential reactivity with hydrophobic molecular patches.
摘要
本笔记描述了四种脂多糖A单克隆抗体（MAb）的结合特异性，包括Centoxin（HA-1A）；这些单克隆抗体表现出相似的结合特性。单克隆抗体与脂多糖A和热灭活的光滑型细菌发生反应，而与光滑型脂多糖（LPS）未观察到反应性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的细菌提取物的免疫印迹显示，单克隆抗体与包括分子量标记物在内的许多多肽条带结合。通过煮沸或二硫苏糖醇处理使牛血清白蛋白（BSA）变性可暴露抗体表位。此外，还观察到与共价偶联到BSA的疏水性脂肪族C12链以及单链DNA的结合。这些克隆的多反应性很可能是由与疏水分子斑块的优先反应性介导的。
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本文引用的文献
1
Anti-endotoxin human monoclonal antibody A6H4C5 (HA-1A) utilizes the VH4.21 gene.
Clin Infect Dis. 1995 Oct;21 Suppl 2:S186-9. doi: 10.1093/clinids/21.supplement_2.s186.
2
Diversity in lipid A binding ligands: comparison of lipid A monoclonal antibodies with rBPI23.
Prog Clin Biol Res. 1995;392:453-63.
3
Reactivity of the human antiendotoxin immunoglobulin M monoclonal antibody HA-1A with lipopolysaccharides from rough and smooth gram-negative organisms.
Infect Immun. 1993 May;61(5):1756-63. doi: 10.1128/iai.61.5.1756-1763.1993.
4
A controlled trial of HA-1A in a canine model of gram-negative septic shock.
JAMA. 1993 May 5;269(17):2221-7.
5
Characterization of specific binding of a human immunoglobulin M monoclonal antibody to lipopolysaccharide and its lipid A domain.
Infect Immun. 1993 Mar;61(3):910-8. doi: 10.1128/iai.61.3.910-918.1993.
6
Antibiotics enhance binding by human lipid A-reactive monoclonal antibody HA-1A to smooth gram-negative bacteria.
Infect Immun. 1993 Feb;61(2):512-9. doi: 10.1128/iai.61.2.512-519.1993.
7
An artificial glycoconjugate containing the bisphosphorylated glucosamine disaccharide backbone of lipid A binds lipid A monoclonal antibodies.
Infect Immun. 1993 Oct;61(10):4514-7. doi: 10.1128/iai.61.10.4514-4517.1993.
8
Immunotherapy of endotoxemia and septicemia.
Immunobiology. 1993 Apr;187(3-5):464-77. doi: 10.1016/S0171-2985(11)80357-8.
9
Treatment of septic shock with human monoclonal antibody HA-1A. A randomized, double-blind, placebo-controlled trial. CHESS Trial Study Group.
Ann Intern Med. 1994 Jul 1;121(1):1-5. doi: 10.7326/0003-4819-121-1-199407010-00001.
10
The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed.
Immunobiology. 1993 Dec;189(5):457-71. doi: 10.1016/S0171-2985(11)80417-1.
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