Cuende J I, Civeira P, Diez N, Prieto J
Laboratorio de Biología Molecular, Hospital Provincial San Telmo, Palencia.
An Med Interna. 1997 Sep;14(9):441-4.
Chronic fatigue syndrome (CFS) is a disorder of unknown etiology. Some viruses have been associated with CFS etiology, specially herpesviruses, enteroviruses and retroviruses. Some studies suggest an association between human herpesvirus-6 (HHV-6) and CFS. In order to know if there is an active HHV-6 infection in CFS patients we studied the immunologic and virologic status of HHV-6.
Twenty patients with CFS were studied. IgG and IgM anti HHV-6 were determined by indirect immunofluorescence assay. DNA from serum and peripheral blood mononuclear cells (PBMC) were studied by dot- and Southern-blotting and nested-PCR to detect HHV-6 DNA. HHV-6 RNA from PBMC were amplified by RT(retrotranscription)-PCR.
Ten patients (50%) had IgG anti-HHV-6 in serum but none had IgM anti-HHV-6. Dot-blotting of DNA from 200 microliters of serum and Southern-blotting of 10 micrograms of PBMC DNA were negative. Nested-PCR from sera were negative. Nested-PCR with 1 microgram PBMC DNA were positive in 8 out 20 (40%) and with 5 micrograms PBMC DNA were positive in 16 out of 20 (80%). No viral RNA were detected in PBMC.
There is a high proportion of CFS patients infected with HHV-6 but with low viral load. Results do not support HHV-6 reactivation in CFS patients.
慢性疲劳综合征（CFS）是一种病因不明的疾病。一些病毒与CFS的病因有关，特别是疱疹病毒、肠道病毒和逆转录病毒。一些研究表明人类疱疹病毒6型（HHV - 6）与CFS之间存在关联。为了了解CFS患者是否存在HHV - 6的活动性感染，我们研究了HHV - 6的免疫和病毒学状态。
对20例CFS患者进行了研究。通过间接免疫荧光法测定抗HHV - 6的IgG和IgM。通过斑点杂交和Southern杂交以及巢式PCR研究血清和外周血单个核细胞（PBMC）中的DNA，以检测HHV - 6 DNA。通过逆转录（RT）-PCR扩增PBMC中的HHV - 6 RNA。
10例患者（50%）血清中有抗HHV - 6的IgG，但无一例有抗HHV - 6的IgM。200微升血清DNA的斑点杂交和10微克PBMC DNA的Southern杂交均为阴性。血清的巢式PCR为阴性。1微克PBMC DNA的巢式PCR在20例中有8例（40%）呈阳性，5微克PBMC DNA的巢式PCR在20例中有16例（80%）呈阳性。在PBMC中未检测到病毒RNA。
有很大比例的CFS患者感染了HHV - 6，但病毒载量较低。结果不支持CFS患者中HHV - 6的重新激活。
