Porter R E, Suckow M A, Macri N P, Bowersock T L
Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA.
Avian Dis. 1997 Oct-Dec;41(4):981-7.
A study was conducted to determine the effective size for latex microsphere uptake in the intestine of white leghorn chickens. Three trials were conducted in which ligated intestinal segments of anesthetized 8-wk-old chickens were injected with 0.2-, 0.5-, 2-, 6-, 10-, or 20-mu diameter fluoresceinated latex microspheres. Microspheres were counted in brush border, epithelium, and lamina propria of each intestinal segment, liver, and spleen. After 1 hr, the 0.2-, 0.5-, and 2-mu microspheres were oriented along the brush border of epithelial cells and microsphere uptake into the epithelium and lamina propria was observed in the duodenum, ileum, cecum, cecal tonsil, and colon. Uptake of microspheres of 6, 10, and 20 mu diameter into epithelium and lamina propria was not observed in any intestinal segment. Also, no microspheres of any diameter were observed in sections of liver and spleen to suggest that there was no appreciable entry of microspheres into the bloodstream within 1 hr after administration. The results indicated that uptake of microspheres by the chicken intestine is a size-dependent process with microspheres < or = 2 mu being taken up to an equal extent by most segments of intestine.
进行了一项研究，以确定白色来亨鸡肠道摄取乳胶微球的有效尺寸。进行了三项试验，向麻醉的8周龄鸡的结扎肠段注射直径为0.2、0.5、2、6、10或20微米的荧光标记乳胶微球。对每个肠段、肝脏和脾脏的刷状缘、上皮和固有层中的微球进行计数。1小时后，0.2、0.5和2微米的微球沿上皮细胞的刷状缘排列，并且在十二指肠、回肠、盲肠、盲肠扁桃体和结肠中观察到微球摄取进入上皮和固有层。在任何肠段均未观察到直径为6、10和20微米的微球摄取进入上皮和固有层。此外，在肝脏和脾脏切片中未观察到任何直径的微球，这表明给药后1小时内微球没有明显进入血液循环。结果表明，鸡肠道对微球的摄取是一个大小依赖性过程，直径≤2微米的微球被大多数肠段以相同程度摄取。
