Aloui R, Pacheco Y, Chabannes B, Gormand F, Piperno D, Biot N, Lagarde M, Perrin-Fayolle M
Laboratoire d'Immuno-Allergologie Respiratoire, Unité INSERM, Centre Hospitalier Lyon-Sud, France.
J Lipid Mediat. 1991 Mar-Apr;3(2):187-96.
Modifications of lipid metabolism and increased plasma membrane fluidity may occur during cell activation and occur in leukocytes of patients with allergic disease. Using a fluorescent probe (TMA-DPH) and a polarization technique, we studied the variations of membrane fluidity in blood leukocytes from allergic and normal subjects. The anisotropic coefficient, which is inversely related to the rotational motion of the probe in membrane phospholipids, was significantly higher in normal subjects than in allergic ones. This result implies an increased membrane fluidity in leukocytes from allergic patients. No correlation could be observed with the etiology or the severity of allergic disease, nor with cell preparation composition or serum IgE level. Such a modification of membrane fluidity has been reproduced after incubation of leukocytes from normal subjects with leukotriene B4, but not with cells from allergic patients. Establishing the mechanism of these differences requires further investigations.
在细胞活化过程中可能会发生脂质代谢的改变以及质膜流动性的增加，这在过敏性疾病患者的白细胞中也会出现。我们使用荧光探针（TMA-DPH）和偏振技术，研究了过敏受试者和正常受试者血液白细胞中膜流动性的变化。各向异性系数与探针在膜磷脂中的旋转运动呈负相关，正常受试者的该系数显著高于过敏受试者。这一结果表明，过敏患者白细胞中的膜流动性增加。未观察到与过敏性疾病的病因、严重程度、细胞制备成分或血清IgE水平存在相关性。在用白三烯B4孵育正常受试者的白细胞后，可重现这种膜流动性的改变，但过敏患者的细胞则不会。要确定这些差异的机制，还需要进一步研究。
