Chang Y W, Hawkins B R
Department of Pathology, University of Hong Kong, Hong Kong.
Hum Immunol. 1997 Aug-Sep;56(1-2):125-35. doi: 10.1016/s0198-8859(97)00108-0.
HLA-A, and -B antigen, gene and haplotype frequencies have been calculated from 18,774 southern Chinese donors on a Hong Kong-based bone marrow donor registry. Two hundred and fifty donors were also tested for HLA-DRB1, -DQA1, and -DQB1 alleles using the PCR-SSP technique. Forty-one HLA-A, -B combinations showed statistically significant positive linkage disequilibrium at the 0.05 level after adjustment for the number of haplotypes theoretically possible and 43 showed significant negative disequilibrium. Thirty-nine different DRB1-DQA1-DQB1 haplotypes were identified of which 20 occurred 5 or more times. The most prevalent was DRB10901-DQA10301-DQB10303 (haplotype frequency = 0.1620). This is the first paper to report the distribution of DQA10104, DQA105011 and DQA105012/13 alleles in Chinese. These data are important for phylogenetic, comparative and medico-legal studies and are of particular value in estimating the likelihood of obtaining appropriately matched donors for Chinese patients awaiting bone marrow transplantation.
已根据香港骨髓捐献者登记处的18774名中国南方捐献者的数据计算出HLA - A、- B抗原、基因及单倍型频率。另外，采用聚合酶链反应-序列特异性引物（PCR - SSP）技术对250名捐献者进行了HLA - DRB1、- DQA1和- DQB1等位基因检测。在对理论上可能的单倍型数量进行校正后，41种HLA - A、- B组合在0.05水平上显示出具有统计学意义的正连锁不平衡，43种显示出显著的负连锁不平衡。共鉴定出39种不同的DRB1 - DQA1 - DQB1单倍型，其中20种出现5次或以上。最常见的是DRB10901 - DQA10301 - DQB10303（单倍型频率 = 0.1620）。本文首次报道了中国人群中DQA10104、DQA105011及DQA105012/13等位基因的分布情况。这些数据对于系统发育、比较及法医学研究具有重要意义，对于评估等待骨髓移植的中国患者找到合适配型捐献者的可能性具有特殊价值。
