Banks J F
Analytica of Branford, Inc., CT 06405, USA.
Electrophoresis. 1997 Nov;18(12-13):2255-66. doi: 10.1002/elps.1150181216.
Successful on-line interfacing of capillary electrophoresis (CE) with electrospray (ES) mass spectrometry (MS) has progressed substantially in recent years. Of particular note also is the development which has occurred in combining the more advanced capillary-based electromigration separation techniques, such as capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CIT), micellar electrokinetic chromatography (MEKC) and capillary electrochromatography (CEC), with ES/MS. The union of these electromigration schemes with MS detection provides a useful and sensitive analytical tool for the separation, quantitation and identification of biological, therapeutic, environmental and other important classes of chemical analytes. By making optimal use of the characteristics inherent with these separation mechanisms, greatly enhanced MS performance may be obtained. The following review summarizes the significant issues and challenges involved with CE/ES/MS analysis as well as results which have recently been obtained.
近年来，毛细管电泳（CE）与电喷雾（ES）质谱（MS）的成功在线联用取得了长足进展。特别值得一提的是，在将更先进的基于毛细管的电迁移分离技术，如毛细管凝胶电泳（CGE）、毛细管等电聚焦（CIEF）、毛细管等速电泳（CIT）、胶束电动色谱（MEKC）和毛细管电色谱（CEC）与ES/MS相结合方面也有了发展。这些电迁移方案与质谱检测的结合为生物、治疗、环境及其他重要化学分析物类别的分离、定量和鉴定提供了一种有用且灵敏的分析工具。通过充分利用这些分离机制所固有的特性，可以获得大大增强的质谱性能。以下综述总结了CE/ES/MS分析所涉及的重大问题和挑战以及最近取得的结果。
