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毛细管电泳-质谱法在尿液中生物重要分析物分析中的当前应用（2017 年至 2021 年年中）：综述。

Current applications of capillary electrophoresis-mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid-2021): A review.

机构信息

Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic.

Faculty of Science, Masaryk University, Brno, Czech Republic.

出版信息

J Sep Sci. 2022 Jan;45(1):305-324. doi: 10.1002/jssc.202100621. Epub 2021 Oct 7.

DOI:10.1002/jssc.202100621
PMID:34538010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9292318/
Abstract

Capillary electrophoresis coupled online with mass detection is a modern tool for analyzing wide ranges of compounds in complex samples, including urine. Capillary electrophoresis with mass spectrometry allows the separation and identification of various analytes spanning from small ions to high molecular weight protein complexes. Similarly to the much more common liquid chromatography-mass spectrometry techniques, the capillary electrophoresis separation reduces the complexity of the mixture of analytes entering the mass spectrometer resulting in reduced ion suppression and a more straightforward interpretation of the mass spectrometry data. This review summarizes capillary electrophoresis with mass spectrometry studies published between the years 2017 and 2021, aiming at the determination of various compounds excreted in urine. The properties of the urine, including its diagnostical and analytical features and chemical composition, are also discussed including general protocols for the urine sample preparation. The mechanism of the electrophoretic separation and the instrumentation for capillary electrophoresis with mass spectrometry coupling is also included. This review shows the potential of the capillary electrophoresis with mass spectrometry technique for the analyses of different kinds of analytes in a complex biological matrix. The discussed applications are divided into two main groups (capillary electrophoresis with mass spectrometry for the determination of drugs and drugs of abuse in urine and capillary electrophoresis with mass spectrometry for the studies of urinary metabolome).

摘要

毛细管电泳与质谱在线联用是分析复杂样品中广泛化合物（包括尿液）的现代工具。毛细管电泳与质谱联用允许从小离子到高分子量蛋白质复合物等各种分析物的分离和鉴定。与更为常见的液相色谱-质谱技术类似，毛细管电泳分离减少了进入质谱仪的分析物混合物的复杂性，从而减少了离子抑制，并使质谱数据分析的解释更为直接。本综述总结了 2017 年至 2021 年间发表的毛细管电泳与质谱联用研究，旨在测定尿液中排泄的各种化合物。还讨论了尿液的性质，包括其诊断和分析特性以及化学成分，包括尿液样品制备的一般方案。还包括电泳分离的机制和毛细管电泳与质谱联用的仪器。本综述表明了毛细管电泳与质谱联用技术在分析复杂生物基质中不同种类分析物的潜力。讨论的应用分为两个主要组（用于尿液中药物和滥用药物测定的毛细管电泳与质谱联用以及用于尿液代谢组学研究的毛细管电泳与质谱联用）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/5c09851ffb8d/JSSC-45-305-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/be64cd4ebde4/JSSC-45-305-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/5c09851ffb8d/JSSC-45-305-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/be64cd4ebde4/JSSC-45-305-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/6939bb7b9c83/JSSC-45-305-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/5d5794aed7fd/JSSC-45-305-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/d7eff29e86c7/JSSC-45-305-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f7/9292318/5c09851ffb8d/JSSC-45-305-g001.jpg

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