ONTRAK TESTCUP、abuscreen ONTRAK、abuscreen ONLINE与气相色谱/质谱联用尿液分析测试结果的比较。

A comparison of ONTRAK TESTCUP, abuscreen ONTRAK, abuscreen ONLINE, and GC/MS urinalysis test results.

作者信息

Crouch D J, Cheever M L, Andrenyak D M, Kuntz D J, Loughmiller D L

机构信息

Center for Human Toxicology, University of Utah, Salt Lake City, USA.

出版信息

J Forensic Sci. 1998 Jan;43(1):35-40.

PMID:9456522

Abstract

This study was designed to compare results obtained from two separate on-site drug testing kits (ONTRAK TESTCUP and Abuscreen ONTRAK) with those obtained from laboratory based immunoassay and GC/MS. Abuscreen ONLINE immunoassay was used to select 250 negative samples and 100 presumptive-positive samples each for cocaine/metabolites, opiates and cannabinoids. Presumptive-positive samples were selected if the immunoassay response was > or = 300 ng/mL for cocaine/metabolites (BZE), > or = 300 ng/mL for opiates or > or = 50 ng/mL for cannabinoids (THC-COOH). GC/MS was used to confirm that each selected sample contained > or = 150 ng/mL BZE, > or = 300 ng/mL morphine/codeine or > or = ng/mL THC-COOH. TESTCUP results had a 100% agreement with GC/MS and a > 99% agreement with ONLINE when testing negative samples. The agreement between TESTCUP and ONLINE results for samples containing opiates was 100%. Results of testing samples containing BZE with TESTCUP demonstrated a 98% agreement with both GC/MS and ONLINE. Both discrepant samples contained BZE at concentrations < or = 300 ng/mL. The least agreement between TESTCUP and ONLINE results was found when testing samples containing THC-COOH. The agreement with ONLINE and GC/MS was 92% and all discrepant samples had GC/MS determined THC-COOH concentrations less than 50 ng/mL. A 100% agreement was obtained between expected and recorded TESTCUP results for QC samples fortified to contained BZE, morphine or THC-COOH at concentrations within 120% of the screening cutoffs. ONTRAK had a 100% agreement with both GC/MS and ONLINE when testing negative samples and samples that contained opiates. ONTRAK had a 91% agreement with GC/MS and ONLINE for testing of samples that contained BZE. The least agreement between ONTRAK and ONLINE results was found when testing samples that contained THC-COOH. The agreement was 89%, however, all discrepant samples contained GC/MS concentrations of THC-COOH less that the 50 ng/mL cutoff. With ONTRAK, a 100% agreement was obtained between expected and recorded results QC samples that contained morphine or THC-COOH and a 97.7% agreement was obtained between expected and recorded results on QC samples that contained BZE.

摘要

本研究旨在比较两种独立的现场药物检测试剂盒（ONTRAK TESTCUP和Abuscreen ONTRAK）与基于实验室的免疫测定法和气相色谱/质谱法（GC/MS）所获得的结果。使用Abuscreen ONLINE免疫测定法分别为可卡因/代谢物、阿片类药物和大麻素选择250份阴性样本和100份推定阳性样本。如果免疫测定反应对于可卡因/代谢物（苯甲酰爱康宁）≥300 ng/mL、对于阿片类药物≥300 ng/mL或对于大麻素（四氢大麻酚酸）≥50 ng/mL，则选择推定阳性样本。使用GC/MS来确认每个选定样本含有≥150 ng/mL苯甲酰爱康宁、≥300 ng/mL吗啡/可待因或≥ ng/mL四氢大麻酚酸。在检测阴性样本时，TESTCUP结果与GC/MS的一致性为100%，与ONLINE的一致性>99%。对于含有阿片类药物的样本，TESTCUP与ONLINE结果之间的一致性为100%。使用TESTCUP检测含有苯甲酰爱康宁的样本，其结果与GC/MS和ONLINE的一致性均为98%。两个有差异的样本中苯甲酰爱康宁浓度均≤300 ng/mL。在检测含有四氢大麻酚酸的样本时，发现TESTCUP与ONLINE结果之间的一致性最低。与ONLINE和GC/MS的一致性分别为92%，所有有差异的样本其GC/MS测定的四氢大麻酚酸浓度均小于50 ng/mL。对于添加了浓度在筛查临界值120%范围内的苯甲酰爱康宁、吗啡或四氢大麻酚酸的质量控制（QC）样本，TESTCUP的预期结果与记录结果之间的一致性为100%。在检测阴性样本和含有阿片类药物的样本时，ONTRAK与GC/MS和ONLINE的一致性均为100%。在检测含有苯甲酰爱康宁的样本时，ONTRAK与GC/MS和ONLINE的一致性为91%。在检测含有四氢大麻酚酸的样本时，发现ONTRAK与ONLINE结果之间的一致性最低。一致性为89%，不过，所有有差异的样本其GC/MS测定的四氢大麻酚酸浓度均低于50 ng/mL临界值。对于ONTRAK，含有吗啡或四氢大麻酚酸的QC样本的预期结果与记录结果之间的一致性为100%，含有苯甲酰爱康宁的QC样本的预期结果与记录结果之间的一致性为97.7%。