对从新生儿大脑中分离出的人N-乙酰-β-D-己糖胺酶C的研究。
Studies on human N-acetyl-Beta-d-hexosaminidase C separated from neonatal brain.
作者信息
Besley G T, Broadhead D M
出版信息
Biochem J. 1976 Apr 1;155(1):205-8. doi: 10.1042/bj1550205.
Abstract
Human brain hexosaminidase C was separated from isoenzymes A and B by Sephadex G-200 gel filtration. Properties of the enzyme were studied, particularly its isoelectric-focusing profile, pI4.80. These findings indicate that hexosaminidase C is identical with the major residual component of Sandhoff fibroblasts with respect to substrate specificity, pI and activity pH optimum.
摘要
通过葡聚糖凝胶G-200凝胶过滤法，从己糖胺酶A和B的同工酶中分离出了人脑海绵状血管瘤己糖胺酶C。对该酶的特性进行了研究，尤其是其等电聚焦图谱，pI为4.80。这些发现表明，就底物特异性、pI和活性最适pH而言，己糖胺酶C与桑德霍夫成纤维细胞的主要残留成分相同。
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