Brattain M G, Kimball P M, Pretiow T G
Cancer Res. 1977 Mar;37(3):731-5.
Isoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of beta-hexosaminidase B than beta-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that beta-hexosaminidase B was more stable to heat and more active at low pH than beta-hexosaminidase A. Kinetic studies revealed that the A and B forms of beta-hexosaminidase had essentially the same Km and Vmax for p-nitrophenyl-N-acetyl-beta-D-glucosaminide.
粗提物的等电聚焦显示，人类结肠癌中β-己糖胺酶B的比例高于β-己糖胺酶A，而正常人类结肠黏膜中该酶的A形式比例更高。对分离出的同工酶的研究表明，β-己糖胺酶B比β-己糖胺酶A对热更稳定，在低pH值下活性更高。动力学研究表明，β-己糖胺酶的A和B形式对对硝基苯基-N-乙酰-β-D-氨基葡萄糖苷的Km和Vmax基本相同。
