Kanik K S, Hagiwara E, Yarboro C H, Schumacher H R, Wilder R L, Klinman D M
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
J Rheumatol. 1998 Jan;25(1):16-22.
To compare patterns of cytokine secretion in patients with new onset synovitis (< 1 yr duration, n = 14), chronic rheumatoid arthritis (RA) (n = 16), and healthy controls (n = 17).
ELIspot assays were used to detect mononuclear cells in the peripheral blood (PMBC) and synovial fluid (SFMC) secretion the type 1 cytokines interleukin 2 (IL-2) and interferon-gamma (IFN-gamma), the type 2 cytokines IL-4, IL-6 and IL-10, and the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). Results were correlated with measures of disease activity.
Patients with new onset synovitis had increased (p < 0.05) numbers of PBMC secreting IL-2. The number of PBMC secreting IFN-gamma correlated with the joint score in the new onset synovitis population (p = 0.006). By comparison, patients with chronic RA had significantly increased numbers of PBMC secreting IL-6, IL-10, and TNF-alpha (p < 0.05). The production of these cytokines correlated with joint score in chronic RA (p = 0.008, 0.06, 0.001, respectively).
Patients with new onset synovitis have increased numbers of PBMC secreting IL-2 and IFN-gamma, while patients with chronic RA have increased numbers of PBMC secreting IL-6, IL-10, and TNF-alpha. Correlations between joint score and number of PBMC secreting cytokines suggest the number of PBMC secreting IFN-gamma is most relevant in new onset synovitis, while the number of PBMC secreting IL-6, IL-10, and TNF-alpha is of greater relevance in chronic RA.
比较新发滑膜炎患者（病程<1年，n = 14）、慢性类风湿关节炎（RA）患者（n = 16）和健康对照者（n = 17）的细胞因子分泌模式。
采用酶联免疫斑点试验（ELIspot）检测外周血单个核细胞（PMBC）和滑液单个核细胞（SFMC）分泌1型细胞因子白细胞介素2（IL-2）和干扰素-γ（IFN-γ）、2型细胞因子IL-4、IL-6和IL-10以及炎性细胞因子肿瘤坏死因子-α（TNF-α）。将结果与疾病活动度指标进行关联分析。
新发滑膜炎患者分泌IL-2的PBMC数量增加（p<0.05）。在新发滑膜炎人群中，分泌IFN-γ的PBMC数量与关节评分相关（p = 0.006）。相比之下，慢性RA患者分泌IL-6、IL-10和TNF-α的PBMC数量显著增加（p<0.05）。这些细胞因子的产生与慢性RA的关节评分相关（分别为p = 0.008、0.06、0.001）。
新发滑膜炎患者分泌IL-2和IFN-γ的PBMC数量增加，而慢性RA患者分泌IL-6、IL-10和TNF-α的PBMC数量增加。关节评分与分泌细胞因子的PBMC数量之间的相关性表明，分泌IFN-γ的PBMC数量在新发滑膜炎中最相关，而分泌IL-6、IL-10和TNF-α的PBMC数量在慢性RA中更相关。
