Sugaya M, Tagawa M, Matsubara H, Gunji Y, Takenaga K, Maeda T, Koide Y, Asano T, Ochiai T, Isono K, Sakiyama S
Department of Surgery (II), School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan.
Int J Oncol. 1998 Feb;12(2):321-4. doi: 10.3892/ijo.12.2.321.
We have examined antitumor effect of human esophageal carcinoma cells (T.Tn) which were retrovirally transduced to express mouse granulocyte macrophage-colony stimulating factor (mGM-CSF) gene. Nude mice inoculated with T.Tn cells secreting mGM-CSF developed small tumors but the tumors regressed spontaneously, although the proliferation in vitro of transduced cells was not different from that of wild-type cells. In contrast, the tumor of T.Tn cells transduced with human GM-CSF grew as that of wild-type cells, since murine GM-CSF receptors do not bind to human GM-CSF. Histological examination of the regressing tumor of mGM-CSF-producing T.Tn cells revealed predominant infiltration of inflammatory cells including macrophages. In addition, local injection of mGM-CSF-producing T.Tn cells into the established wild-type tumors significantly induced the retardation of subsequent wild-type tumor growth, suggesting that T-cell independent local response plays a crucial role in destroying tumor cells.
我们检测了通过逆转录病毒转导以表达小鼠粒细胞巨噬细胞集落刺激因子（mGM-CSF）基因的人食管癌细胞（T.Tn）的抗肿瘤作用。接种分泌mGM-CSF的T.Tn细胞的裸鼠形成了小肿瘤，但肿瘤随后自发消退，尽管转导细胞的体外增殖与野生型细胞并无差异。相比之下，转导人GM-CSF的T.Tn细胞的肿瘤生长情况与野生型细胞相同，因为小鼠GM-CSF受体不与人GM-CSF结合。对产生mGM-CSF的T.Tn细胞消退肿瘤的组织学检查显示，包括巨噬细胞在内的炎性细胞大量浸润。此外，将产生mGM-CSF的T.Tn细胞局部注射到已形成的野生型肿瘤中，可显著诱导后续野生型肿瘤生长的延缓，这表明不依赖T细胞的局部反应在破坏肿瘤细胞中起关键作用。
